Research on Differences Between Main Secondary Metabolites and Molecules of

HAN Duo; ZHAO Zhi-lian; LIU Wei-hong; LI Hai-feng

doi:10.13422/j.cnki.syfjx.2016180035

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Research on Differences Between Main Secondary Metabolites and Molecules of

更新时间：2024-01-04

- Research on Differences Between Main Secondary Metabolites and Molecules of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 18, Pages: 35-39(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016180035
  CLC： O657.72;R284
- Published：2016
- 稿件说明：
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HAN Duo, ZHAO Zhi-lian, LIU Wei-hong, et al. Research on Differences Between Main Secondary Metabolites and Molecules of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 35-39.
DOI：

HAN Duo, ZHAO Zhi-lian, LIU Wei-hong, et al. Research on Differences Between Main Secondary Metabolites and Molecules of [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 35-39. DOI： 10.13422/j.cnki.syfjx.2016180035.

摘要

目的：通过含量测定与DNA条形码分析，探讨坚龙胆主要次生代谢产物及分子的差异。方法：采用 HPLC 测定不同居群坚龙胆根及根茎、茎、叶、花中主要次生代谢产物龙胆苦苷、獐芽菜苦苷、獐芽菜苷及苦龙胆酯苷含量，建立其指纹图谱共有模式，用单因素方差分析对主要次生代谢产物间的差异进行评价；并采用 DNA 条形码技术对不同居群坚龙胆样品进行 ITS2 测序。结果：不同居群坚龙胆根及根茎、茎、叶、花中龙胆苦苷、獐芽菜苦苷、獐芽菜苷及苦龙胆酯苷含量具有极显著差异（P<0.01）；同时ITS2序列结果显示不同居群坚龙胆分子差异较小。结论：不同居群坚龙胆主要次生代谢产物有一定差异，但分子间差异较小，亲缘关系较近。

Abstract

Objective: To investigate the differences between main secondary metabolites and molecules of Gentiana rigescens from different populations by the method of content determination and DNA barcoding analysis. Method: An HPLC method was applied to determine the contents of gentiopicroside

swertiamarin

sweroside and amarogentin in G. rigescens roots and rhizomes

stems

leases

and flowers

and the HPLC fingerprint was established

the main secondary metabolites were evaluated by one-way ANOVA

and the DNA barcoding technique was used to test ITS2 sequences of G. rigescens. Result: There were significant differencedifferences in the contents of gentiopicroside

swertiamarin

sweroside and amarogentin (P<0.01) in different parts.fractions. The results of ITS2 sequences showed that there was no significant difference in the molecule of G. rigescens from different populations. Conclusion: There was differences in main secondary metabolites of G. rigescens from different populations

but there was little difference of with less differences among molecules and a close genetic relationship.

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Related Institution

Nanyang Vocational College of Agriculture

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Dongfang Hospital， Beijing University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Key Laboratory of Standardization of Chinese Medicine，State Key Laboratory of Southwestern Chinese Medicine Resource ，School of Pharmacy，Chengdu University of Traditional Chinese Medicine（TCM）

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