Compare to Antioxidant Activity of  Flower, Leaf and Stem

LIU Dong-shun; CHEN Chun-lin; CHE Xiao-hang; TANG Lin-yan; CHEN Li; XU Li-li; XIONG Xiao-juan

doi:10.13422/j.cnki.syfjx.2016180040

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Compare to Antioxidant Activity of Flower, Leaf and Stem

更新时间：2024-01-04

- Compare to Antioxidant Activity of Flower, Leaf and Stem
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 18, Pages: 40-44(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016180040
  CLC： R284
- Published：2016
- 稿件说明：
移动端阅览
LIU Dong-shun, CHEN Chun-lin, CHE Xiao-hang, et al. Compare to Antioxidant Activity of Flower, Leaf and Stem[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 40-44.
DOI：

LIU Dong-shun, CHEN Chun-lin, CHE Xiao-hang, et al. Compare to Antioxidant Activity of Flower, Leaf and Stem[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 40-44. DOI： 10.13422/j.cnki.syfjx.2016180040.

摘要

目的：研究菊花、叶和茎的体外抗氧化活性，为菊花、叶、茎资源的充分利用提供科学依据。方法：以绿原酸为对照组（CHA），分为菊花水提物组（JH-S），菊花醇提物组（JH-C），菊叶水提物组（JY-S），菊叶醇提物组（JY-C），菊花茎水提物组（JJ-S），菊花茎醇提物组（JJ-C），分别测定提取物的还原能力及对羟基自由基（·OH），二苯代苦味酰基自由基（DPPH·），脂自由基和超氧阴离子（O2-·）的清除率，研究其体外抗氧化活性。结果：菊花，叶，茎提取物和绿原酸的抗氧化作用与浓度呈正相关。菊花、叶、茎的水、醇提取物（0.20 g·L-1）和绿原酸相比，还原能力分别为2.260，2.270，2.261，2.260，2.261，2.272，2.256均无差异，且各醇提物和水提物之间也无差异；对·OH，O2-·均具较强的清除作用，且水提物的作用均好于其醇提物，尤其抗O2-·作用明显强于绿原酸清除DPPH·，脂自由基方面效果均优于水提物，菊茎醇提取物对DPPH·清除率略高于绿原酸，相同浓度下菊叶清除脂自由基作用胜于菊花。结论：菊花、叶和茎提取物均有较强的抗氧化活性，菊叶抗氧化作用与菊花相当。菊叶和茎作为天然抗氧化剂有较好的开发利用价值。

Abstract

Objective: To study the antioxidant activity of Chrysanthemum morifolium flowers

leaves and stems in vitro. Method: Chlorogenic acid was used for the control group (CHA). Extractives were extracted from C. morifolium flowers

leaves and stems. Water and ethanol were used as solvents. These extracts were divided into six groups: flower water extracts (JH-S) group; flower alcohol extracts (JH-C) group

leaf water extracts (JY-S) group

leaf alcohol extracts (JY-C) group

stem water extracts (JJ-S) group; and stem alcohol extracts (JJ-C) group. The antioxidant activity of different extracts were studied by determining the reducing capacity

and the clearance rates of hydroxyl (·OH) radical

1-diphenyl-2-pieryl-2-picrylhydrazyl (DPPH·) radical

lipid free radical and superoxide anion (O2-·) radical. The antioxidant activity of chlorogenic acid was compared with that of the extracts. Result: The anti-oxygenation of extracts from C. morifolium flowers

leaves and stems have positively correlated with the concentration

which was the same as chlorogenic acid. There had no difference between ethanol extracts and water extracts from C. morifolium flowers

leaves and stems in reducing capacity; the ethanol extracts and water extracts show a high efficiency in clearing·OH

O2-·radical

and water extracts had a better efficiency than ethanol extracts

with an obviously better effect in resisting O2-·than chlorogenic acid. Ethanol extracts of C. morifolium flowers and leaves were better than its water extracts in cleaning DPPH and lipid free radical ability. The efficiency in clearing DPPH·of ethanol extracts from C. morifolium stems was higher than that of chlorogenic acid. The cleaning lipid free radical function of extracts from C. morifolium leaves was better than that of extracts from C. morifolium flowers with the same concentration. Conclusion: Extracts of C. morifolium flowers

leaves and stems shows an efficient antioxidant activity. The anti-oxygenation function of leaves was equal with flowers. As natural antioxidants

C. morifolium leaves and stems have a great development and utilization value in the future.

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