Objective: To prepare the solid phase extraction column for the effective separation and enrichment of glycyrrhizic acid. Method: A novel surface molecularly imprinted polymer was synthesized

with acrylamide(AM)-modified multi-walled carbon nanotubes(MWCNTs) as supporting matrix

glycyrrhizic acid as template molecule

acrylamide(AM) as functional monomer

N

N-dimethyl formamide(DMF) as pore former and ethylene glycol dimethacrylate(EGDMA) as cross-linking agent. The multi-walled carbon nanotubes molecularly imprinted polymers were characterized by FTIR

SEM and TGA. Result: A stable even layer was imprinted on the MWCNTs surface when the precipitation polymerization temperature was 60℃

the pore forming agent was DMF

EGDMA and solvent ratio was 1:20.The Scatchard model shows that MWCNTs-molecularly imprinted polymers(MIP) had two kinds of binding sites with different affinities and specific recognition ability of glycyrrhizic acid(Kd 1.17 mmol·L-1

Qmax 741.5 μg·g-1

Kd 3.96 mmol·L-1

Qmax 1 668.5 μg·g-1). Conclusion: The molecularly imprinted polymers synthesized with optimal conditions have a good morphological structure and adsorption efficiency

so these polymers as solid-phase extraction material have a certain research value in separation and enrichment of glycyrrhizic acid in medicinal herbs.