Determination and Pharmacokinetic Analysis of Phenolic Acids and Flavonoids After Oral Administration of Shuanghuanglian Preparation by UPLC-MS/MS

LIU Ting; DI Liu-qing; LI Juan; KANG An; LI Jun-song; ZHAO Xiao-li; SHAN Jin-jun

doi:10.13422/j.cnki.syfjx.2016180082

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Determination and Pharmacokinetic Analysis of Phenolic Acids and Flavonoids After Oral Administration of Shuanghuanglian Preparation by UPLC-MS/MS

更新时间：2024-01-04

- Determination and Pharmacokinetic Analysis of Phenolic Acids and Flavonoids After Oral Administration of Shuanghuanglian Preparation by UPLC-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 18, Pages: 82-87(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016180082
  CLC： R284;R285
- Published：2016
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LIU Ting, DI Liu-qing, LI Juan, et al. Determination and Pharmacokinetic Analysis of Phenolic Acids and Flavonoids After Oral Administration of Shuanghuanglian Preparation by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 82-87.
DOI：

LIU Ting, DI Liu-qing, LI Juan, et al. Determination and Pharmacokinetic Analysis of Phenolic Acids and Flavonoids After Oral Administration of Shuanghuanglian Preparation by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(18): 82-87. DOI： 10.13422/j.cnki.syfjx.2016180082.

摘要

目的：建立同时测定大鼠灌服双黄连制剂后血浆中酚酸类（新绿原酸、绿原酸及隐绿原酸）与黄酮类（黄芩苷、千层纸素A-7-O-β-D-葡萄糖苷、汉黄芩苷、黄芩素、汉黄芩素及千层纸素A）成分的超高效液相色谱-串联质谱法（UPLC-MS/MS），并研究其药代动力学。方法：血浆样品采用乙酸乙酯-正丁醇（1：1）萃取，流动相0.4%甲酸水溶液-[乙腈-甲醇（4：1）混合液]梯度洗脱，流速0.4 mL·min-1，采用电喷雾离子化，多反应监测离子扫描模式，选用响应较高的正离子模式。结果：建立的同时测定血浆中9种成分的UPLC-MS/MS线性良好（R2>0.992），精密度RSD均<14.2%，准确度85.49%～114.76%，复方中新绿原酸、绿原酸、隐绿原酸、黄芩苷、千层纸素A-7-O-β-D-葡萄糖苷、汉黄芩苷、黄芩素的药时曲线下面积AUC0-t与AUC0-∞与单味药提取物相比显著增加，而酚酸和黄酮类成分的消除速率较单味药提取液缓慢。结论：建立的UPLC-MS/MS特异、快速、准确及灵敏，可用于双黄连制剂中9种主要活性成分在大鼠体内的药代动力学研究。

Abstract

Objective: A rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry method (UPLC-MS/MS) was developed and validated for simultaneously determination of phenolic acids and flavonoids in rat plasma after oral administration of Shuanghuanglian preparation. Method: Plasma samples were pretreated by n-butanol-ethyl acetate(1:1).The separation was preformed on an Waters Acquity HSS T3 C18 column(2.1 mm×100 mm

1.7 μm) and the flow rate was set at 0.4 mL·min-1 with mobile phase of acetonitrile-methanol(4:1)-0.4% formic acid for gradient elution.The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring(MRM) via electrospray ionization(ESI) source with positive mode. Result: All calibration curves had good linearity(R2>0.992).RSDs of precision and accuracy were all satisfied.After oral administration of Shuanghuanglian preparation

a remarkable increase in values of AUC0-t and AUC0-∞ of neochlorogenic acid

chlorogenic acid

cryptochlorogenic acid

baicalin

oroxylin A-7-O-β-D-glucuronide

wogonoside and baicalein was observed by compared with Lonicerae Japonicae Flos extract group or Scutellariae Radix extract group;but the elimination rate of phenolic acids and flavonoids in Shuanghuanglian preparation was slower than the single herb extract. Conclusion: The developed method is successfully applied to pharmacokinetic study of nine active ingredients in Shuanghuanglian preparation

this study can provide experimental evidence for elucidating rationality of compatibility of this preparation.

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