Objective: To define the type of fungi causing Gardenia jasminoides pathogenic anthracnose

and study the inhibitory effect of commonly antisepticson the mycelial growth of the pathogens

in order to provide a basis for preventing and treating the disease. Method: G.jasminoides anthracnose leaves were collected from several regions of Jiangxi province. The pathogens were isolated and purified by general tissue separation. According to the Koch's postulates

pathogenicity test

pathogen morphological observation

microscopic identification and rDNA-ITS sequence analysis were used to identify the pathogens. Eleven fungicides were selected to test their indoor sterilizing effect. Thetoxicity regression equationfor agent concentration and inhibition ratio was established

and the effective fungicides were screened out

in a bid to provide a theoretical basis for controlling the disease. Result: Eleven strains were separated from G.jasminoides anthracnose leaves. Through pathogenic determination and Koch's Postulates

one strain was confirmed to be the main pathogen that attacks G.jasminoides quickly and severely. On the basis of morphology and microscopic identification

and Blast comparison analysis for rDNA-ITS sequences

the pathogenic strain was identified to be Phoma.sp. The fungicides Wuyiencin

streptomycin and oligosaccharide chain protein had no significant inhibition effect

but carbendazol showed the best sterilizing effect

whose regression equation was Y=79.97X-1.75 (r=0.965

EC50=0.65 mg·L-1). The next were prochloraz tricyclazole and pentanitro-carbendazim

whose regression equations were Y=6.142X+19.47(r=0.902

EC50=4.97 mg·L-1) and Y=6.1X+17.23(r=0.93

EC50=5.37 mg·L-1)

respectively. The others showed varying inhibition effect. Conclusion: The G. jasminoides anthrax pathogen was determined

and effective agents to inhibit pathogens were screened out.