Content Determination of Sesquiterpene Coumarin (DAW22) in  by UPLC

ZHU Yun; ZHANG Ke; LIANG Xu; TAN Yong; HUANG Jian; WANG Jin-hui

doi:10.13422/j.cnki.syfjx.2016190063

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Content Determination of Sesquiterpene Coumarin (DAW22) in by UPLC

更新时间：2024-01-04

- Content Determination of Sesquiterpene Coumarin (DAW22) in by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 19, Pages: 63-65(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016190063
  CLC： O657.72;R284.1
- Published：2016
- 稿件说明：
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ZHU Yun, ZHANG Ke, LIANG Xu, et al. Content Determination of Sesquiterpene Coumarin (DAW22) in by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 63-65.
DOI：

ZHU Yun, ZHANG Ke, LIANG Xu, et al. Content Determination of Sesquiterpene Coumarin (DAW22) in by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 63-65. DOI： 10.13422/j.cnki.syfjx.2016190063.

摘要

目的：利用超高效液相色谱法测定了分布于新疆的不同生长时期多伞阿魏中DAW22的含量。方法：采用固相萃取（SPE）净化，测定条件Waters ACQUITY UPLC BEH C18色谱柱（2.1 mm×50 mm，1.7 μm），以甲醇-1%甲酸水溶液为流动相，进行梯度洗脱，流速0.2 mL·mL-1，柱温30℃，波长316 nm，进行含量测定。结果：DAW22进样量在6.21～124.2 ng（r=1.000 0）与其峰曲线下面积呈良好的线性关系，平均回收率99.81%，RSD 2.0%，生长在5月9日的多伞阿魏植株中DAW22的含量最高。结论：建立的多伞阿魏中DAW22的质量控制研究方法准确、可靠、重复性好，能够用于多伞阿魏的质量控制。

Abstract

Objective: To establish UPLC method for the determination of DAW22 from Ferula ferulaeoides of different growth periods in Xinjiang. Method: Solid phase extraction(SPE) was used for purification

and the determination was performed on ACQUITY UPLC BEH C18(2.1 mm×50 mm

1.7 μm) with methanol-1% formic acid solution as the mobile phase for gradient elution. The flow rate was 0.2 mL·min-1. Column temperature was 30℃ and UV detection wavelength was 316 nm. Result: The sample volume showed good linear relationship with area under the peak curve within the range of 6.21-124.2 ng(r=1.000 0) for DAW22. The average recovery was 99.81%

and the RSD was 2.0%. The content of DAW22 was highest for those grown in May 9th. Conclusion: This method is accurate

simple and reproducible

and can be applied to the quality control of F. ferulaeoides.

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Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

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Third Affiliated Hospital of Henan University of Chinese Medicine

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Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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