LIU Jian-qun, WANG Zhuo, XU Jin-di, et al. Effect of Glycyrrhizae Radix et Rhizoma on Metabonomics of Triptonide[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 70-75.
DOI:
LIU Jian-qun, WANG Zhuo, XU Jin-di, et al. Effect of Glycyrrhizae Radix et Rhizoma on Metabonomics of Triptonide[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 70-75. DOI: 10.13422/j.cnki.syfjx.2016190070.
Effect of Glycyrrhizae Radix et Rhizoma on Metabonomics of Triptonide
Objective: To investigate effect of Glycyrrhizae Radix et Rhizoma on metabonomics of triptonide
find toxicity biomarkers and attenuation biomarkers induced by Glycyrrhizae Radix et Rhizoma and disclose toxicity and attenuation mechanism of Glycyrrhizae Radix et Rhizoma on triptonide. Method: SD rats were randomly divided into three groups of the control group
the triptonide group (dose of 0.7 mg·kg-1) and the triptonide (dose of 0.7 mg·kg-1)+Glycyrrhizae Radix et Rhizoma (dose of 3 mL·kg-1) group.All drug treatments were administered daily for consecutive 7 days.All urine samples were determined by UPLC-Q-TOF-MS.These obtained data were analyzed by principal component analysis (PCA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA). Result: Seven biomarkers related to toxicity of triptonide were found
four of them were identified as pantothenic acid
kynurenic acid
tryptophan and coproporphyrin
respectively.Four attenuation biomarkers were identified
one of them was tryptophan
but these remaining three biomarkers were unknown. Conclusion: These toxicity biomarkers and attenuation biomarkers are identified for the first time.It is indicated that toxicity of triptonide interfered with the energy
tryptophan and porphyrin metabolisms in body
recover of these metabolisms induced by Glycyrrhizae Radix et Rhizoma may be a physiological attenuation mechanism of Glycyrrhizae Radix et Rhizoma on triptonide.
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