Effect of Nitidine Chloride on Inhibiting MCF-7 Cell Proliferation and Effect on P125 Expression

LIAO Liu-feng; OU Xian-hong; LIU Hua-gang; WU Qiong

doi:10.13422/j.cnki.syfjx.2016190085

您当前的位置：

首页 >

文章列表页 >

Effect of Nitidine Chloride on Inhibiting MCF-7 Cell Proliferation and Effect on P125 Expression

更新时间：2024-01-04

- Effect of Nitidine Chloride on Inhibiting MCF-7 Cell Proliferation and Effect on P125 Expression
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 19, Pages: 85-89(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016190085
  CLC： R285
- Published：2016
- 稿件说明：
移动端阅览
LIAO Liu-feng, OU Xian-hong, LIU Hua-gang, et al. Effect of Nitidine Chloride on Inhibiting MCF-7 Cell Proliferation and Effect on P125 Expression[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 85-89.
DOI：

LIAO Liu-feng, OU Xian-hong, LIU Hua-gang, et al. Effect of Nitidine Chloride on Inhibiting MCF-7 Cell Proliferation and Effect on P125 Expression[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 85-89. DOI： 10.13422/j.cnki.syfjx.2016190085.

摘要

目的：研究氯化两面针碱（NC）对体外培养的乳腺癌细胞（MCF-7）中DNA聚合酶δ催化亚基P125（P125）表达的影响，探讨氯化两面针碱对乳腺癌细胞增殖抑制作用的机制。方法：设定NC（0.075，0.15，0.30，0.60，1.20，2.40，4.80 mg·L-1）组及空白组，应用噻唑蓝（MTT）比色法测定不同质量浓度NC对MCF-7细胞的生长抑制率；通过细胞克隆集落形成实验进一步验证NC对MCF-7细胞的生长抑制作用；实时荧光定量PCR（Real-time PCR）检测低、中、高质量浓度NC（0.5，1.0，2.0 mg·L-1）对P125编码基因DNA聚合酶δ催化亚基基因（POLD1），p53基因（p53），细胞周期依赖性蛋白激酶抑制因子p21（p21）mRNA表达水平的影响；蛋白质免疫印迹法（Western blot）检测3种质量浓度NC对P125蛋白表达的影响，使用酶联免疫吸附法（ELISA）测定p53，p21蛋白表达变化。结果：NC对MCF-7细胞有明显的抑制作用，呈浓度依赖性，不同质量浓度NC（0.075，0.15，0.30，0.60，1.20，2.40，4.80 mg·L-1）对细胞的抑制率分别是0.16%，6.29%，19.56%，32.17%，44.46%，73.46%，83.21%。与空白组比较，NC对细胞克隆集落形成有显著抑制作用（P<0.01），不同质量浓度NC组对POLD1 mRNA表达有明显的抑制作用，同时明显降低P125蛋白表达（P<0.05）；对p53 mRNA和蛋白的表达有明显的促进作用（P<0.01）；高剂量NC组对p21 mRNA和蛋白的表达有显著增强作用（P<0.01）。结论：NC能明显抑制乳腺癌MCF-7细胞的增殖，其作用机制之一可能与上调p53表达水平，抑制P125表达有关。

Abstract

Objective: To study the effect of nitidine chloride (NC) on expression of DNA polymerase δ catalytic subunit P125 in breast cancer cells (MCF-7 cells)

and investigate the mechanism of NC on inhibiting proliferation of breast cancer cells. Method: Different concentrations (0.075

0.15

0.3

0.60

1.20

4.80 mg·L-1) NC groups and blank group were established. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was applied to evaluate the inhibitory rate of cells after treatment with different concentrations of NC. At the same time

the clone form assay was used to further verify the inhibitory effect of different concentrations of NC on MCF-7 cells proliferation

the effects of different concentrations of NC (0.50

1.00

2.00 mg·L-1) on mRNA expressions of DNA polymerase δ catalytic subunit gene POLD1

p53 and p21 were quantified by Real-time PCR. The protein expression of P125 was detected by Western blot

and the protein expressions of p53 and p21 were detected by enzyme linked immunosorbent assay (ELISA). Result: NC had obvious inhibitory effect on MCF-7 cells in a concentration-dependent manner. NC with concentrations of 0.075

0.15

0.3

0.60

1.20

2.40

4.80 mg·L-1 had an inhibitory rate of 0.16%

6.29%

19.56%

32.17%

44.46%

73.46%and 83.21%respectively on the cells. As compared with the blank group

NC had significant inhibitory effect on the formation of cell colonies (P<0.01)

in addition

the mRNA expression of POLD1 and the protein expression of P125 in various NC groups were significantly decreased (P<0.05)

the mRNA and protein expressions of p53 were significantly up-regulated (P<0.01)

but the mRNA and protein expressions of p21 were significantly up-regulated only in the high dose NC group (P<0.01). Conclusion: NC can significantly inhibit the proliferation of MCF-7

and one of the mechanisms may be associated with up-regulating the level of p53 expression and inhibiting the expression of P125.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Haizao Yuhutang Combined with Modified Sargassum and Glycyrrhizae Radix et Rhizoma on Expression of p53/p21/Caspase-3 Pathway in Rats with Goiter

Effect of Huluan Decoction on SIRT1/NF-κB/p53/p21 Pathway in Model Mice with Premature Ovarian Insufficiency

Role and Mechanism of Cucurbitacin B in Suppressing Proliferation of Breast Cancer 4T1 Cells via Inducing Ferroptosis

Mechanism of Polyphyllin Ⅱ in Induction of Ferroptosis in Hepatocellular Carcinoma HepG2 Cells

Related Author

TIAN Yi

DONG Xiao

LIU Xiaoqing

CAO Can

WU Meijing

XU Xiangnan

LIAO Wenyong

CHEN Shaohong

Related Institution

Beijing University of Chinese Medicine

College of Integrated Traditional Chinese and Western Medicine， Hunan University of Chinese Medicine

The Second Military Medical University

Hunan Key Laboratory of Traditional Chinese Medicine Prescription and Syndromes Translational Medicine，Hunan University of Chinese Medicine

School of Chinese Material Medica， Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰