Essential Oil from Aucklandiae Radix Induces Apoptosis and Potential Mechanism in MV4-11 Leukemia Cells

YANG Yu-ting; HE Yu-lin; HE Bei-xuan; XIONG Liang; CAO Zhi-xing; PENG Cheng

doi:10.13422/j.cnki.syfjx.2016190095

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Essential Oil from Aucklandiae Radix Induces Apoptosis and Potential Mechanism in MV4-11 Leukemia Cells

更新时间：2024-01-04

- Essential Oil from Aucklandiae Radix Induces Apoptosis and Potential Mechanism in MV4-11 Leukemia Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 19, Pages: 95-99(2016)
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- DOI：10.13422/j.cnki.syfjx.2016190095
  CLC： R285
- Published：2016
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YANG Yu-ting, HE Yu-lin, HE Bei-xuan, et al. Essential Oil from Aucklandiae Radix Induces Apoptosis and Potential Mechanism in MV4-11 Leukemia Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 95-99.
DOI：

YANG Yu-ting, HE Yu-lin, HE Bei-xuan, et al. Essential Oil from Aucklandiae Radix Induces Apoptosis and Potential Mechanism in MV4-11 Leukemia Cells[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 95-99. DOI： 10.13422/j.cnki.syfjx.2016190095.

摘要

目的：以人急性髓性白血病MV4-11细胞为研究对象，探讨木香挥发油对MV4-11细胞增殖与凋亡抑制作用及其作用机制。方法：以质量浓度分别为3，6，12，25，50，100 mg·L-1的木香挥发油作用于人急性髓性白血病MV4-11细胞不同时间，另设空白组，采用噻唑蓝（MTT）法检测木香挥发油对其增殖抑制作用，Hoechst 33258染色法观察细胞凋亡的形态学变化，Annexin V-FITC，PI双染及PI单染法应用流式细胞术检测细胞凋亡和周期，蛋白质免疫印迹（Western blot）检测蛋白激酶B（AKT），半胱氨酸天冬氨酸蛋白酶3（Caspase-3）凋亡蛋白的表达。结果：木香挥发油对MV4-11细胞的半抑制浓度（IC50）为13.33 mg·L-1；木香挥发油作用于MV4-11细胞24 h后可使细胞核皱缩，染色质凝聚，形成明显的凋亡小体；与空白组比较，木香挥发油G0/G1期和G2/M期细胞明显减少，S期细胞明显增多（P<0.05，P<0.01）；与空白组比较，12，25，50，100，150 mg·L-1木香挥发油能诱导MV4-11细胞凋亡（P<0.05）；与空白组比较，25，50，100，150 mg·L-1木香挥发油可抑制AKT的磷酸化，并促进Caspase-3蛋白的降解（P<0.05，P<0.01）。结论：木香挥发油能抑制人白血病细胞MV4-11细胞的增殖，其作用机制可能与抑制AKT活性进而诱导细胞凋亡效应有关。

Abstract

Objective: To investigate the effect of essential oil from Aucklandiae Radix on proliferation and apoptosis inhibition of MV4-11 leukemia cells and discuss its action mechanism. Method: Human leukemia cell line MV4-11 was treated with the essential oil from Aucklandiae Radix (3

100 mg·L-1) for different time

and another blank group was set up. Cell viability was estimated by using MTT assay; the apoptosis morphology changes were observed by Hoechst 33258 staining; flow cytometry was used on Annexin V-FITC/propidium iodide staining to detect the cell cycle and the rate of apoptosis; Western blot was used to detect AKT and Caspase-3 protein expression. Result: The essential oil from Aucklandiae Radix had IC50 value of 13.33 mg·L-1; various nuclear changes such as nuclear shrinkage

chromatin condensation and obvious apoptotic bodies were observed after MV4-11 cell line was treated with essential oil from Aucklandiae Radix for 24 h. As compared with the blank group

the cells in G0/G1 phase were significantly reduced and the cells in S phase were significantly increased with the increase from essential oil from Aucklandiae Radix oil dosage (P<0.05

P<0.01). 12

100

150 mg·L-1 essential oil from Aucklandiae Radix induced apoptosis of MV4-11 (P<0.05); 25

100

150 mg·L-1 essential oil from Aucklandiae Radix inhibited AKT phosphorylation and promoted Caspase-3 protein degradation (P<0.05

P<0.01). Conclusion: The essential oil from Aucklandiae Radix can inhibit the proliferation of MV4-11 cells in vitro

and the mechanism may be correlated with inducing cell apoptosis by inhibiting AKT activity.

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