Effects of Bushen Tiaochong Prescription on Expression of Sex Hormone and Bcl-2/Bax in Ovaries of Rats with Premature Ovarian Failure

LIANG Ce; GAO Hui; ZHANG Teng

doi:10.13422/j.cnki.syfjx.2016190100

您当前的位置：

首页 >

文章列表页 >

Effects of Bushen Tiaochong Prescription on Expression of Sex Hormone and Bcl-2/Bax in Ovaries of Rats with Premature Ovarian Failure

更新时间：2024-01-04

- Effects of Bushen Tiaochong Prescription on Expression of Sex Hormone and Bcl-2/Bax in Ovaries of Rats with Premature Ovarian Failure
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 19, Pages: 100-104(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016190100
  CLC： R285.5
- Published：2016
- 稿件说明：
移动端阅览
LIANG Ce, GAO Hui, ZHANG Teng. Effects of Bushen Tiaochong Prescription on Expression of Sex Hormone and Bcl-2/Bax in Ovaries of Rats with Premature Ovarian Failure[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 100-104.
DOI：

LIANG Ce, GAO Hui, ZHANG Teng. Effects of Bushen Tiaochong Prescription on Expression of Sex Hormone and Bcl-2/Bax in Ovaries of Rats with Premature Ovarian Failure[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(19): 100-104. DOI： 10.13422/j.cnki.syfjx.2016190100.

摘要

目的：观察补肾调冲方对雷公藤多苷片所致卵巢早衰（premature ovarian failure，POF）雌性大鼠卵巢性激素水平和卵巢中B细胞淋巴瘤/白血病-2（B cell lymphoma/leukemia-2，Bcl-2），促进凋亡蛋白Bcl-2相关X蛋白（Bcl-2 assaciated X protein，Bax）蛋白及mRNA表达的影响，探讨补肾调冲方的预防作用。方法：SD雌性大鼠42只，随机分为正常组，模型组，结合雌激素组，补肾调冲方预防高、中、低剂量组。运用酶联免疫吸附试验（ELISA）法检测血清中雌二醇（estradiol，E2），卵泡刺激素（follicule-stimulating hormone，FSH），黄体生成素（luteinizing hormone，LH），孕酮（progesterone，P）水平。蛋白质免疫印迹（Western blot）法和逆转录-聚合酶链式方应（RT-PCR）法检测卵巢组织中Bcl-2，Bax蛋白和mRNA的表达水平。结果：与正常组比较，模型组大鼠FSH，LH含量明显升高，E2的含量明显降低，卵巢组织中Bcl-2蛋白及mRNA的表达明显降低，Bax蛋白及mRNA的表达明显升高（P<0.01）；补肾调冲方各给药组和结合雌激素组E2的含量均高于模型组（P<0.01），其中补肾调冲方低、中、高剂量和结合雌激素组E2含量与正常组比较，差异无统计学意义。补肾调冲方各给药组和结合雌激素组FSH，LH的含量均低于模型组（P<0.01）；低、中、高剂量组和结合雌激素组FSH，LH含量与正常组比较，差异无统计学意义。各组与正常组P含量比较差异无统计学意义。补肾调冲方预防低、中、高剂量组和结合雌激素组Bcl-2蛋白及mRNA的表达明显高于模型组（P<0.01）；Bax蛋白及mRNA的表达明显低于模型组（P<0.01）。结论：补肾调冲方对卵巢具有一定的保护作用，可以预防卵巢早衰的发生。其作用机制可能与补肾调冲方中某些中药能够调节卵巢性激素水平，维持Bcl-2，Bax的平衡，降低卵泡闭锁的速度，抑制卵泡的过度凋亡，改善卵巢的功能。

Abstract

Objective: To investigate the effect of Bushen Tiaochong prescription on protein and mRNA expression levels of sex hormone

B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in ovaries of rats with premature ovarian failure(POF) induced by triptergium wilfordii (GTW)

and explore the prevention effect of Bushen Tiaochong prescription. Method: Forty-two healthy female SD rats were randomly divided into normal group

model group

estrogen group

Bushen Tiaochong prescription low

middle and high dose groups. 17-estradiol (E2)

follicle-stimulating hormone (FSH)

luteinizing hormone (LH) and progesterone (P) levels in serum were detected by enzyme-linked immunosorbent assay (ELISA). Expression levels of Bcl-2 and Bax protein and mRNA in rats ovarian were detected by Western blot and RT-PCR methods. Result: As compared with the normal group

FSH and LH contents were significantly increased in model group rats; E2 contents were significantly decreased; Bcl-2 protein and mRNA expression levels in ovarian were significantly decreased; while Bax protein and mRNA expression levels were significantly increased (P<0.01). E2 content in Bushen Tiaochong prescription groups and estrogen group was higher than that in model group (P<0.01)

but middle

high dose groups and estrogen group had no statistically significant differences in E2 as compared with the normal group. The FSH and LH contents in Bushen Tiaochong prescription groups and estrogen group were lower than those in model group(P<0.01)

and there were no statistically significant differences as compared with normal group. All dose groups had no significant difference with normal group in P content. Bcl-2 protein and mRNA expression levels in middle and high dose Bushen Tiaochong prescription groups and estrogen group were significantly higher than those in model group (P<0.01); Bax protein and mRNA expression levels were significantly lower than those in model group(P<0.01). Conclusion: It is indicated that Bushen Tiaochong prescription has some protective effects for ovaries and prevents POF. It can regulate sex hormone level

maintain Bcl-2 and Bax balance to decrease follicles atresia and death speed

and improve ovarian function.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Bushen Huoxue Prescription in Regulating PINK1/Parkin Pathway in Rat Model of Premature Ovarian Failure

Zuoguiwan Treats Cyclophosphamide-induced Premature Ovarian Failure by Regulating SLC7A11/GPX4 Pathway to Inhibit Ferroptosis

Mechanism of Cuscutae Semen to Control Premature Ovarian Failure Based on Lipid and Atherosclerosis Pathways

Mechanism of Jiawei Bazhen Yimu Capsule in Treatment of Premature Ovarian Failure Based on Estrogen Signaling Pathway

Effect of Ranae Oviductus on Follicular Development Based on PI3K/Akt Signaling Pathway

Related Author

WANG Kailing

JING Yichen

WANG Guiyun

LI Yueheng

LIU Huiping

ZHAO Min

ZHANG Jinrong

HUA Jianghuan

Related Institution

School of Integrated Chinese and Western Medicine， School of Chinese Medicine， The Second Affiliated Hospital， School of Medicine， and Hunan Provincial Key Laboratory of Translational Medicine in Traditional Chinese Medicine Formula-Syndrome Research， Hunan University of Chinese Medicine

School of Basic Medical Sciences， Hubei University of Chinese Medicine

Hubei Shizhen Laboratory

Experimental Center of Chinese Medicine， Hubei University of Chinese Medicine

Experimental Center of Traditional Chinese Medicine，Hubei University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰