Objective: To search small molecule inhibitors for HIV-1 intergrase from traditional Chinese medicine database platform (TCMSP) by using the virtual screening technology. Method: The interacting site between HIV-1 intergrase andcytokineLEDGF/P75 were taken as target. The molecular docking technology was used for the first round of screening

then the ADME/T prediction was adopted for the second round of screening

and finally the target point andthe interacting site were based for the third round of screening. Result: The free binding energy of original ligand (4-[(5-bromo-4-{[2

4-dioxo-3-(2-oxo-2-phenylethyl)-1

3-thiazolidin-5-ylidene]methyl}-2-ethoxyphenoxy)-methyl]-benzoic acid

D77) was be used as positive control to screen out two natural micro-molecule compounds with good drug likeness. Thenatural micro-molecule compounds

HIV-1 intergraseandinteractive perssad showed a superioraffinity to D77 (a new-typeintergrase inhibitor). Their sources of traditional Chinese medicine were determined. Conclusion: This study successfully established a high-throughput virtual screening strategy for HIV-1 intergrase inhibitors

and provides an important reference and theoretical basis for the extraction of anti-AIDS compounds from Chinese herbal medicine and the design of anti-AIDS drugs.