Objective: To establish an HPLC method for the simultaneous determination of four flavonoids components in flowers of Coreopsis tinctoria. Method: The chromatographic separation was performed on Cosmosil-5C18-AR-Ⅱ column (4.6 mm×250 mm

5 μm) with acetonitrile-0.1% acetic acid solution as the mobile phase for gradient elution

at a flow rate of 1.0 mL·min-1

and the detection wavelength was set at 280 nm (for flavanomarein

isookanin) and 378 nm (for marein

okanin) to determine the contents of flavanomarein

isookanin

marein and okanin in Coreopsis tinctoria. Result: 4 flavonoids components were well separated

with good linear relationship between 0.503 5-5.035 μg

0.502 5-5.025 μg

0.505 5-5.055 μg

0.506 5-5.065 μg

r>0.999

and average recovery rate was between 95.4%-98.4%. Conclusion: The method is accurate and selective

which could provide reference for the quality control of C. tinctoria.