Objective: To screen out compounds with estrogen-like effects from Epimedii Folium by liquid chromatography/mass spectrometry technology and molecular docking for breast cancer T47D cell. Method: The T47D cells were cultured by medium with Epimedii Folium extract

and the compounds with estrogen-like effects selectively bound with the cells. After culturing for a certain period of time

T47D cells were cleaned and broken to release the binding compounds. Then high performance liquid chromatography/mass spectrometry (HPLC-TOF/MS) technology was used to identify the binding compounds. After that

molecular docking was used to study the interactions between the identified binding compounds and estrogen receptors. Result: Seven compounds from Epimedii Folium were identified preliminarily

namely icariin

epimedin B

baohuoside-Ⅱ

icariin-I

baohuoside-I

korepimedoside A and anhydroicaritin. Among them

baohuoside-Ⅱ and anhydroicaritin can interact with estrogen receptor-α and estrogen receptor-β. Conclusion: Compounds baohuoside-Ⅱ

and anhydroicaritin can be bound with T47D cells and interact with estrogen receptor-α and estrogen receptor-β

which indicates that baohuoside-Ⅱ and anhydroicaritinmay have estrogen-like effects.