Objective: To observe the effect of different doses Kangxianling decoction on the expression of PI3K-AKT-mTOR mRNA in mice with chronic renal failure induced by 5/6 nephrectomized

and discuss its action mechanism. Method: Totally 50 C57BL/6J mice were selected in the chronic renal fibrosis model induced by 5/6 nephrectomy

with 10 rats in the sham-operated group. After the successful modeling

the rats were divided into the model group

the rapamycin group (0.8 mg·kg-1)

Kangxianling decoction low

middle and high-dose groups (10

20

40 g·kg-1)

ig

for eight weeks. After the rats were sacrificed

HE staining was used to observe the renal pathological changes and the score of Kidney damage was made

qPCR was made to detect PI3K-AKT-mTOR mRNA expression. Result: The renal tissue fibrosis

the score of kidney damage and the PI3K-AKT-mTOR mRNA expression in model group were higher than those in the sham group

with statistically significant differences(P<0.01); compared with the model group

the renal tissue fibrosis

the score of kidney damage and the PI3K-AKT-mTOR mRNA expression decreased in RAP group and all of Kangxianling decoction groups significantly decreased (P<0.05

P<0.01); compared with the RAP group

the middle and high-dose groups had no significant difference and the low-dose group showed a lower efficacy (P<0.05); compared with the low-dose group

middle and high-dose groups had significant differences (P<0.05)

but there were no significant difference between middle and high-dose groups. Conclusion: Kangxianling decoction could reduce the score of kidney damage and PI3K-AKT-mTOR mRNA expression

and the middle and high-dose Kangxianling decoctions have the same effect as RAP.