Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats

DU Li-dong; WU Guo-tai; WANG Xia; ZHANG Xiu-xiu; SHAO Jing; WANG Rui-qiong; REN Yuan

doi:10.13422/j.cnki.syfjx.2016200112

您当前的位置：

首页 >

文章列表页 >

Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats

更新时间：2024-01-04

- Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 20, Pages: 112-116(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016200112
  CLC： R285.5
- Published：2016
- 稿件说明：
移动端阅览
DU Li-dong, WU Guo-tai, WANG Xia, et al. Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 112-116.
DOI：

DU Li-dong, WU Guo-tai, WANG Xia, et al. Effect of Danggui Buxue Tang on Peritoneal Function Failure Induced by Peritoneal Dialysis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(20): 112-116. DOI： 10.13422/j.cnki.syfjx.2016200112.

摘要

目的：研究当归补血汤（Danggui Buxue Tang，DBT）对腹膜透析相关性大鼠腹膜功能衰竭的影响，并探讨相关的作用机制。方法：SD大鼠ip 4.25%高糖透析液100 mL·kg-1，连续40 d，在第8，10，12天ip大肠埃希菌脂多糖5 mg·kg-1，建立腹膜功能衰竭大鼠模型；造模大鼠随机分为4组，每组10只，分别为模型组，贝那普利组（2.1 mg·kg-1），DBD高、低剂量组（12，6 g·kg-1），另设正常组，各组大鼠造模的同时ig给药；实验期间观察各大鼠活动状态，第40天末次ip透析液2 h后进行腹膜平衡实验，计算超滤量（UF），检测血清肌酐（PCr）和尿素氮（PUN），腹透液中肌酐（DCr），尿素氮（DUN），葡萄糖（DGlu）及4.25%透析液中的葡萄糖（D0Glu），计算腹膜转运功能参数（DCr/PCr，DUN/PUN，DGlu/D0Glu），取大鼠壁层腹膜采用苏木素-伊红（HE）观察组织形态变化，并用免疫组化法检测转化生长因子-β1（TGF-β1）蛋白的表达。结果：与正常组比较，模型组大鼠PCr和PUN均显著升高（P<0.01），DCr和DGlu显著降低，DUN显著升高（P<0.01），UF，DCr/PCr，DUN/PUN及DGlu/D0Glu均显著降低（P<0.01），腹膜间皮细胞受损明显，腹膜TGF-β1表达增高（P<0.01）；与模型组比较，DBT高、低剂量组PCr和PUN均显著降低（P<0.05），DGlu显著升高（P<0.01），DBT低剂量组DUN显著升高（P<0.05），DBT高剂量组大鼠UF显著升高（P<0.05），DBT高、低剂量组DCr/PCr，DUN/PUN及DGlu/D0Glu均显著升高（P<0.01）；DBT高、低剂量组大鼠腹膜组织形态明显改善，且TGF-β1表达显著降低（P<0.05，P<0.01）。结论：DBT能抑制腹膜透析相关性大鼠腹膜功能衰竭，保护腹膜结构。

Abstract

Objective: To observe the protective effects of Danggui Buxue Tang (DBT) on peritoneal function failure induced by peritoneal dialysis in rats. Method: Peritoneal function failure model in rats were established by intraperitoneally injecting 4.25%of high-glucose peritoneal dialysate (100 mL·kg-1) for 40 d and administering lipopolysaccharide (LPS

5 mg·kg-1) at 8

12 d. The rats were randomly divided into four groups

with 10 rats in each group. They were respectively model group

Benazepril Hydrochloride group (BH 2.1 mg·kg-1)

DBT high-dose and low-dose groups (12

6 g·kg-1). The other 10 rats were randomly chosen as normal control group. During modeling

rats in each group were given drugs

ig. During the experiment

body weights and general states of all rats were detected. At 40 d

the two hour later after intraperitoneal administration with dialysate

the peritoneal equilibration test was performed to detect the ultrafiltration volume (UF

PCr

PUN

DCr

DUN and DGlu)

and calculate peritoneal transport function parameters (DCr/PCr

DUN/PUN

DGlu/D0Glu). Parietal peritoneum tissues of rats were stained with hematoxylin-eosin (HE) to observe the changes of peritoneal morphology

the transforming growth factor-β1(TGF-β1) expression in parietal peritoneum of rats WAS observed by Immunohistochemical method. Result: Compared with normal group

in the model group

PCr

PUN

DUN were increased (P<0.01)

the levels of DCr

DGlu

DCr/PCr

DUN/PUN and DGlu/D0Glu were decreased (P<0.01)

peritoneal mesothelioma cells were significantly impaired

and the expression of TGF-β1 was also increased (P<0.01). Compared with model group

in DBT high-dose and low-dose groups

PCr

PUN were decreased (P<0.05)

the levels of DGlu were significantly increased (P<0.01)

DCr/PCr

DUN/PUN and DGlu/D0Glu were significantly increased (P<0.05

P<0.01)

with obvious improvement in peritoneal histomorphology and decrease in TGF-β1 expression (P<0.05

P<0.01). Conclusion: DBT shows the protective effects on peritoneal function failure induced by peritoneal dialysis in rats.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research Progress on Pharmacodynamic Material Basis and Quality Control of Danggui Buxue Tang

Protective Effect of Danggui Buxue Tang on Impaired Functional Activity of Endothelial Cells Exposed to Low Flow Shear Stress

Effect of Yiqi Yangyin Zhuyu Recipe in Anti-inflammation Activities in Vitro on LPS-induced RAW 264.7 Cells

Molecule Mechanism of Fushen Granules in Improving Peritoneal Ultrafiltration Function

Related Author

Hong-hua WU

Jia-yu TONG

Zhao-jie MA

Yun-tao DAI

Rong SUN

Xiao-tian QI

Ru-na JIN

Zhen QIN

Related Institution

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Institute of Advanced Medical Sciences, School of Pharmaceutical Sciences, Shandong University

The Second Hospital of Shandong University

Tianjin Key Laboratory of Modern Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine

School of Basic Medicine, Guizhou Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰