Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis
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Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis
Chinese Journal of Experimental Traditional Medical FormulaeVol. 22, Issue 21, Pages: 27-31(2016)
YU Hai-shuai. Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 27-31.
DOI:
YU Hai-shuai. Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 27-31. DOI: 10.13422/j.cnki.syfjx.2016210027.
Research on Spectrum-activity Relationship of Echinopsis Radix for Anti-gastric Cancer Based on Principal Component Analysis, Cluster Analysis and Canonical Correlation Analysis
Objective: To study on spectrum-activity relationship between chemical composition and anti-gastric cancer efficacy of Echinopsis Radix in Yuzhou. Method: Chemical composition in Echinopsis Radix from seven regions was analyzed by HPLC
and principal component analysis was carried out for its comom peak
according to correlation coefficient matrix
characteristic value and accumulated variance contribution rate
principal components of Echinopsis Radix were inferred.MTT was used to assay proliferation inhibition rate of Echinopsis Radix from seven regions for gastric cancer cell line SGC7901 and AGS
then for cluster analysis.Canonical correlation analysis was used to analyze relationship between chemical composition and anti-gastric cancer efficacy of Echinopsis Radix. Result: Principal components of Echinopsis Radix were rosmarinic acid
oleanolic acid and stigmasterol in seventeen common peaks.Proliferation inhibition rates of Echinopsis Radix from different region on SGC-7901 and AGS cell were in the order of Henan >Shandong >Liaoning >Shanxi >Anhui >Jiangsu >Gansu.Cluster analysis showed that Henan and Shandong classified the same species
Liaoning and Shanxi classified the same species
Anhui
Jiangsu and Gansu classified the same species. Conclusion: Echinopsis Radix from Henan has the strongest proliferation inhibition effect on gastric cancer cell line SGC7901 and AGS.Rosmarinic acid
oleanolic acid and stigmasterol in Echinopsis Radix may be these principal components for anti-gastric cancer.
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