Objective: To study the extraction

separation and preliminary structural characterization of the polysaccharide from the leaves of Panax notoginseng. Method: Single factor experiment and orthogonal experiment were adopted to optimize the extraction process of the polysaccharide from the leaves of P. notoginseng. Several methods such as resin method

ion exchange chromatography and gel permeation chromatography were used to purify and separate the polysaccharide. Paper chromatography and GC-MS were used to analyze the monosaccharide composition and the backbone structure of the polysaccharides. Result: The optimum extraction process was obtained as follows:extraction temperature of 80℃

extraction time of 1 h

solid to liquid ratio of 1:12

and extraction times of 3 times. Homogeneous polysaccharide A1 was obtained after purification and separation

mainly composed of arabinose

glucose and galactose with the molar ratio of 1.00:1.56:1.17.The backbone chain was mainly composed of glucose and galactose and the branched chain was composed of arabinose and galactose. Conclusion: Hot water extraction technology of the polysaccharides from the leaves of P. notoginseng was optimized. Resin method was used to replace the traditional decolorization and deproteinization methods of polysaccharide

with good effect. In addition

monosaccharide composition of the polysaccharide from the leaves of P. notoginseng was consistent with that of the polysaccharide from the roots of P. notoginseng. The present paper provided theoretical and experimental basis for comprehensive utilization of the polysaccharide from P. notoginseng.