UPLC/Q-TOF-MS Rapid Analysis of Components in Plasma from Guizhi Fuling Capsules

YIN Quan-wei; LI Jia-chun; QIN Jian-ping; HUANG Wen-zhe; WANG Zhen-zhong

doi:10.13422/j.cnki.syfjx.2016210083

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UPLC/Q-TOF-MS Rapid Analysis of Components in Plasma from Guizhi Fuling Capsules

更新时间：2024-01-04

- UPLC/Q-TOF-MS Rapid Analysis of Components in Plasma from Guizhi Fuling Capsules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 21, Pages: 83-86(2016)
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- DOI：10.13422/j.cnki.syfjx.2016210083
  CLC： O657.63;R286.0
- Published：2016
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YIN Quan-wei, LI Jia-chun, QIN Jian-ping, et al. UPLC/Q-TOF-MS Rapid Analysis of Components in Plasma from Guizhi Fuling Capsules[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 83-86.
DOI：

YIN Quan-wei, LI Jia-chun, QIN Jian-ping, et al. UPLC/Q-TOF-MS Rapid Analysis of Components in Plasma from Guizhi Fuling Capsules[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 83-86. DOI： 10.13422/j.cnki.syfjx.2016210083.

摘要

目的：采用UPLC/Q-TOF-MS对桂枝茯苓胶囊入血成分进行定性分析，为该制剂的体内药效物质研究提供参考。方法： Poroshell 120 EC-C18色谱柱（3 mm×100 mm，1.7 μm），流动相乙腈（A）-0.1%甲酸水溶液（B）梯度洗脱（0~5 min，5% A；5~20 min，5%~17% A；20~28 min，17%~30% A；28~30 min，30%~58% A；30~40 min，58%~83% A；40~45 min，83%~88% A；45~50 min，88% A），流速0.4 mL·min-1，柱温35℃，进样量10 μL。采用电喷雾离子源，负离子扫描，毛细管电压3.5 kV，雾化气压力45 Pa，干燥气流速10 L·min-1，加热毛细管温度350℃，源内裂解电压145 V，质量数扫描范围m/z 100~1 200。结果：通过与空白生物样品和对照品的提取离子色谱图、质谱碎片裂解信息比对，初步鉴定出14个入血成分，包括13个原型成分和1个代谢产物（野樱苷）。结论：建立的UPLC/Q-TOF-MS可为桂枝茯苓胶囊入血成分的定性分析提供一种快速、简便、可靠的分析手段，为该制剂的药效物质基础研究奠定基础。

Abstract

Objective: To analyze and identify the chemical components in plasma from Guizhi Fuling capsules by UPLC/Q-TOF-MS. Method: Poroshell 120 EC-C18 column (3 mm×100 mm

1.7 μm) was used for isolation and identification of chemical components in Guizhi Fuling capsules with a mobile phase of methanol-water containing 0.1%formic acid for gradient elution and the flow rate of 0.4 mL·min-1.Electrospray ion (ESI) source were applied for the qualitative analysis under the negative ion mode. Result: Based on UPLC/Q-TOF-MS analysis

a total of 14 compounds (including 13 prototypes and 1 metabolites) were identified by compared with ion chromatograms and pyrolysis information of mass fragmentation of the blank biological samples and reference substances. Conclusion: This method is rapid

simple and credible for identification of ingredients in plasma from Guizhi Fuling capsules

and it can provide a foundation for the illumination of its pharmacodynamics material basis.

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