Objective: To observe and the influence of bufadienolides on the immune-related cytokines in vivo

analyze the effect of drug on immune system regulation

and provide the pharmacological and pharmacodynamics evidences to clarify its immune mechanism. Method: After the AsPC-1 pancreatic cancer tumor cells were cultured in vitro

the animal models of AsPC-1 pancreatic cancer were established in nude mice. After administration for a certain time

flow cytometry with high throughput and multi-factor detection technology was used to detect the cytokines of granulocyte-macrophage colony stimulating factor (GM-CSF)

tumor necrosis factor-α(TNF-α)

interferon-γ(IFN-γ)

interleukin-1α(IL-1α)

interleukin-2 (IL-2)

interleukin-4 (IL-4)

interleukin-5 (IL-5)

interleukin-6 (IL-6) and interleukin-10 (IL-10) in mice serum. Western blot assay was used to detect the protein content of corresponding cytokines in tissues

and its immune effect in vivo was evaluated. Result: In the observation of the drug's immune effect on pancreatic cancer tumor-bearing nude mice in vivo

as compared with gemictabine and cinobufotalin injection

bufadienolides can enhance more cytokine expression. The low dosage group had protective effect on spleen while high dosage group achieved the same effect on spleen as gemictabine and cinobufotalin injection. Conclusion: Bufadienolides can lead to secretion of various immune-related cytokines

enhance the body's immune function

but the specific mechanism of immune function still needs further research.