Objective: To evaluate the effects of Zuogui Wan and Yougui Wan and their disassembled prescriptions on adipocyte-induced bone marrow mesenchymal stem cells(BMSCs) of ovariectomized rat

and discuss their relevant action mechanism. Method: Ninety female rats were selected; 10 of them were randomly selected as the normal group; among the remaining 80 rats

10 were randomly selected to excide a small amount of fat tissue around ovaries through bilateral lumbar incisions as the sham group

while the other 70 were selected to excide bilateral ovaries as the model group. They were divided into nine groups

namely control group

sham group

ovariectomized group

Zuogui Wan group (18.9 g·kg-1)

Yougui Wan group (20.52 g·kg-1)

common prescription group (15.12 g·kg-1)

Yin prescription group (12.96 g·kg-1)

Yang prescription group (8.1 g·kg-1) and progynova group

and simultaneously intervened the BMSCs. The BMSCs were identified by flow cytometry

and lipid droplets of BMSCs were assessed by oil red O staining. The mRNA expression of peroxisome proliferator-activated receptor γ(PPARγ)

lipoprotein (LPL) and fatty acid binding protein 4 (FABP4) were assayed by RT-PCR. The protein expression of CCAAT/enhancer binding protein β(C/EBPβ) was assayed by Western blot. Result: Compared with control group

ovariectomized group showed significant differentiation from BMSCs to adipocytes and increases in PPARγ

LPL

FABP4 mRNA expressions and C/EBPβ protein expression(P<0.05). Compared with ovariectomized group

both Zuogui Wan and Yougui Wan and their disassembled prescriptions can inhibit adipogenic differentiation. Especially

Yougui Wan can remarkablely down-regulate PPARγ

LPL

FABP4 mRNA

and reduce the expression of C/EBPβ protein (P<0.05). Conclusion: Zuogui Wan and Yougui Wan and their disassembled prescriptions can inhibit adipogenic differentiation. Yuogui Wan may have a better effect in inhibiting onadipogenic differentiation than Zuogui Wan

Yang prescription and common prescription.