Protection Effect of Panax Notoginseng Saponins in Rats with Cisplatin-induced Nephrotoxicity

QIU Yue; YANG Yu-fang; HUANG Zhen-guang; ZHOU Jin-ling; LIANG Xue-yan; HUANG Li; CAI Zheng-wen

doi:10.13422/j.cnki.syfjx.2016210104

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Protection Effect of Panax Notoginseng Saponins in Rats with Cisplatin-induced Nephrotoxicity

更新时间：2024-01-04

- Protection Effect of Panax Notoginseng Saponins in Rats with Cisplatin-induced Nephrotoxicity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 21, Pages: 104-109(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016210104
  CLC： R285.5
- Published：2016
- 稿件说明：
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QIU Yue, YANG Yu-fang, HUANG Zhen-guang, et al. Protection Effect of Panax Notoginseng Saponins in Rats with Cisplatin-induced Nephrotoxicity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 104-109.
DOI：

QIU Yue, YANG Yu-fang, HUANG Zhen-guang, et al. Protection Effect of Panax Notoginseng Saponins in Rats with Cisplatin-induced Nephrotoxicity[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 104-109. DOI： 10.13422/j.cnki.syfjx.2016210104.

摘要

目的：应用血清代谢组学方法探讨三七总皂苷（PNS）对早期的顺铂肾损害大鼠的保护作用。方法：顺铂组大鼠单剂量腹腔注射顺铂，PNS干预组大鼠注射顺铂后再腹腔注射PNS，每天1次。24 h后，评价大鼠肾功能，苏木精-伊红染色（HE）观察肾脏组织病理学的改变，并使用核磁共振代谢组学技术进行血清代谢组学的检查。结果： PNS可降低血清肌酐（SCr），血尿素氮（BUN）的浓度和改善肾组织病理损伤。另外，血清标本的主成分分析（PCA），偏最小二乘法判别分析（PLS-DA）和正交偏最小二乘法判别（OPLS-DA）分析显示，顺铂模型组，PNS干预组均与正常组有显著的区分，而顺铂模型组与PNS干预组之间有部分重叠。与正常组比较，顺铂模型组和PNS干预组的丙酮，n-乙酰糖蛋白信号，丙酮酸，脂质和极低密度脂蛋白（VLDL）表达降低，而葡萄糖、缬氨酸和酪氨酸的表达升高。而且，顺铂模型组的乳酸表达较PNS干预组高。另外，正常组和PNS干预组大鼠苯丙氨酸的表达较顺铂模型组高，而酪氨酸的表达较顺铂模型组低。差异代谢物涉及的代谢通路主要有戊糖磷酸途径、糖酵解/糖异生途径、丙酮酸途径等与能量代谢相关的通路。结论： PNS对顺铂肾毒性有保护作用，其保护机制可能和逆转顺铂肾损害引起的代谢紊乱尤其是能量代谢障碍有关。

Abstract

Objective: To investigate the protective effects of panax notoginseng saponins(PNS) in rats with early stage of cisplatin-induced nephrotoxicity through serum metabonomic analysis. Method: The rats received intraperitoneal injection with a single dose of cisplatin

and a subset of rats were also intraperitoneally injected with PNS once a day. After 24 hours

the renal functions of the rats were evaluated; the pathological changes of renal tissues were observed by using hematoxylin-eosin staining (HE); and the serum metabolic profiles were analyzed by using nuclear magnetic resonance-based metabonomics technology. Result: PNS decreased the concentration of serum creatinine (SCr) and blood urea nitrogen (BUN)

and improved the renal histopathological damages. Additionally

principal component analysis (PCA)

partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) of the serum samples showed a clear difference for the normal control group from cisplatin-only group and the cisplatin-PNS group

and showed a part overlap between the cisplatin-only group and the cisplatin-PNS group. Moreover

acetone

n-acetyl glycoprotein signals

pyruvate

lipid and very low density lipoprotein (VLDL) expression levels were lower

while glucose

valine and tyrosine expression levels were higher in the cisplatin-only group and the cisplatin-PNS group when compared with the normal control group. The expression level of lactate in the cisplatin-only group was higher than that in the cisplatin-PNS group. In addition

the expression level of phenylalanine in cisplatin-PNS group and normal control group was higher

while the expression level of tyrosine was lower than those in the cisplatin-only group. Metabolic pathways of different metabolites mainly included pentose-phosphate pathway

glycolysis/gluconeogenesis pathways

pyruvate pathways and other pathways related with energy metabolism. Conclusion: PNS had a protective effect on cisplatin-induced nephrotoxicity

and its protective mechanism may be associated with reversing the disturbed metabolism induced by cisplatin

especially the disturbed energy metabolism.

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