Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome

LIU Yao; JIAO Hao-yan; LIU Wei

doi:10.13422/j.cnki.syfjx.2016210142

您当前的位置：

首页 >

文章列表页 >

Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome

更新时间：2024-01-04

- Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 21, Pages: 142-146(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016210142
  CLC： R285.5
- Published：2016
- 稿件说明：
移动端阅览
LIU Yao, JIAO Hao-yan, LIU Wei. Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 142-146.
DOI：

LIU Yao, JIAO Hao-yan, LIU Wei. Protective and Regulative Effect Patchouli Oil on Intestinal Mucosal Mechanical Barrier and Immune Barrier of Rats with Post-infectious Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 142-146. DOI： 10.13422/j.cnki.syfjx.2016210142.

摘要

目的：研究广藿香油对感染后肠易激综合征（PI-IBS）大鼠肠黏膜机械屏障和免疫屏障的保护作用。方法：将SD大鼠随机分为正常组，PI-IBS模型组、藿香正气液组、广藿香油低、中、高剂量组，每组8只。采用结肠灌注乙酸的方法建立PI-IBS大鼠模型。正常组和模型组ig生理盐水，藿香正气液组ig藿香正气液3.3 mL·kg-1，广藿香油低、中、高剂量组分别ig广藿香油2，3，4 g·kg-1，每日1次，共5 d。采用透射电镜观察结肠黏膜上皮细胞超微结构；采用酶联免疫吸附测定（ELISA）的方法检测各组大鼠血清二胺氧化酶（DAO）和血清免疫球蛋白A（SIgA）含量；采用免疫组化检测结肠肠黏膜细胞间黏附分子-1（ICAM-1）的表达。结果：PI-IBS大鼠结肠上皮细胞微绒毛稀疏，长短不一，有微绒毛断裂现象。广藿香油高剂量组对受损的上皮细胞有改善作用。PI-IBS组血清DAO含量高于正常组（P<0.05）。广藿香油高剂量组血清DAO含量低于PI-IBS组（P<0.05），与正常组比较，无显著性差异。PI-IBS组血清SIgA含量低于正常组（P<0.01）。藿香正气液组、广藿香油高剂量组血清SIgA含量与正常组之间无显著性差异。PI-IBS组ICAM-1蛋白表达IA值明显高于正常组（P<0.01）。广藿香油高剂量组ICAM-1蛋白表达IA值均明显低于模型组（P<0.05），与正常组比较，无显著性差异。结论：广藿香油通过修复受损的肠上皮细胞的超微结构，降低肠道通透性，保护肠黏膜机械屏障。通过促进SIgA分泌，抑制ICAM-1的表达，发挥对PI-IBS免疫屏障的调节作用。

Abstract

Objective: To protective and regulative effect Patchouli oil on intestinal mucosal mechanical barrier and immune barrier of rats with post-infectious irritable bowel syndrome(PI-IBS). Method: Sprague-Dawley rats were randomly divided into normal control group

PI-IBS model group

Huoxiang Zhengqi liquid group (HXZQ group)

low-dose of Patchouli oil

middle-dose of Patchouli oil and high-dose of Patchouli oil

with 8 rats in each group. The rat model of intracolonic instillation of acetic acid (4%)-induced colitis is set up. Control group and PI-IBS model group were treated with water

HXZQ group was treated with HXZQ(3.3 mL·kg-1). Low-dose of Patchouli oil

middle-dose of Patchouli oil and high-dose of Patchouli oil were treated with Patchouli oil (2

4 g·kg-1). After different treatment for 5 days

intestinal epithelial ultrastructure was analyzed by transmission electron microscopy (TEM)

the diamine oxidase (DAO) activity and SIgA content in serum was measured by ELISA assay. ICAM-1 were detected by immunohistochemistry. Result: Microvilli on the surface of the epithelial cells was sparse

uneven distribution and different length in PI-IBS model group. Recovery effect is obvious in high-dose group of Patchouli oil. Serum DAO content in model group was higher than it of the normal group (P<0.05). Compared with PI-IBS model group

serum DAO content in high-dose groups were significantly decreased (P<0.05). The difference between the normal group and high-dose groups was not statistically significant. Serum SIgA content in model group was lower than it of the normal group (P<0.01). The difference between the normal group and high-dose groups or HXZQ group was not statistically significant. ICAM-1 protein expression IA values in model group was higher than it of the normal group (P<0.01). Compared with PI-IBS model group

ICAM-1 protein expression IA values in high-dose groups were significantly decreased (P<0.05). The difference between the normal group and high-dose groups was not statistically significant. Conclusion: Patchouli oil can repair intestinal epithelial ultrastructure in rats with PI-IBS

reduce intestinal permeability

and then protect Intestinal mucosal mechanical barrier. Patchouli oil could promote SIgA

inhibit the expression of ICAM-1

thereby regulate immune barrier of rats with PI-IBS.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical Efficacy of Niuhuang Chengqitang in Treatment of Acute Gastrointestinal Injury in Patients with Severe Traumatic Brain Injury and Its Effect on MTL， DAO， and NSE

Effects of Huayutongmai Injection on Endothelin-1,Nitric Oxide,Intercellular Adhesion Molecule-1 of Rat Aorta after Balloon Injury

Effects of on Renal and Urinary ICAM-1 Expression in Unilateral Ureteral Obstruction Rats

Effects of Didang Decoction on Expression of Intercellular Adhesion Molecular-1 in Diabetic Nephropathy Rats

Related Author

HE Jinbo

MAO Zhengrong

AI Shen

WANG Zhan-wu1

MAO Jing-yuan1

ZHANG Yu1

LI Tan1

LI Ming2

Related Institution

First Affiliated Hospital of Henan University of Chinese Medicine

Nayang Institute of Techonlogy

Hunan University of traditional Chinese medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰