Application of Vacuum Liquid Chromatography in Enrichment and Preparation of Active Ingredients of Traditional Chinese Medicines

YAO Li; LIU Xiao-qian; GAO Hui-min; WANG Zhi-min

doi:10.13422/j.cnki.syfjx.2016210197

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Application of Vacuum Liquid Chromatography in Enrichment and Preparation of Active Ingredients of Traditional Chinese Medicines

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- Application of Vacuum Liquid Chromatography in Enrichment and Preparation of Active Ingredients of Traditional Chinese Medicines
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 21, Pages: 197-202(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016210197
  CLC： O657.7;R284.1
- Published：2016
- 稿件说明：
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YAO Li, LIU Xiao-qian, GAO Hui-min, et al. Application of Vacuum Liquid Chromatography in Enrichment and Preparation of Active Ingredients of Traditional Chinese Medicines[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 197-202.
DOI：

YAO Li, LIU Xiao-qian, GAO Hui-min, et al. Application of Vacuum Liquid Chromatography in Enrichment and Preparation of Active Ingredients of Traditional Chinese Medicines[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(21): 197-202. DOI： 10.13422/j.cnki.syfjx.2016210197.

摘要

中药化学研究日趋关注微量成分、新成分的分离和鉴定，而其生物活性如何、是否具有开发价值等问题，往往止步于其量微难得。现有的制备型HPLC，工业色谱等设备经济性差，难以胜任实验室规模的制备，如何突破该瓶颈的制约，是中药研究能否深入的关键之一。结合本实验室的经验，本文介绍一种高效、便捷、廉价的传统分离技术--真空柱色谱法，尽管它已广泛应用于制药、食品、化妆品等行业，但缘于该技术诀窍缺乏系统披露，对于初涉中药研究领域的学生或科研工作者来说，大量制备目标化合物仍是一种挑战。本文结合实例，在简述真空柱色谱特点、原理、操作要点的基础上，着重介绍了该技术在新化合物发现、已知化合物快速制备中的应用，并介绍应用该技术进行制备过程中的实验室分离策略。

Abstract

With the development of traditional Chinese medicine(TCM) chemistry

more and more studies have focused on the separation and identification of micro-components and new components. However

their biological activity and development value have been hard to be studied due to the difficulties in enrichment and purification. Although

great progresses had been achieved in chromatography technology

such as HPLC and preparative HPLC

it is still hard to prepare in a large scale in laboratory. In order to find a suitable technique

vacuum liquid chromatography (VLC)

which is an effective

convenient and cheap traditional separation technique

was recommended according to our experiences in reference examples during the preparation of these target compounds. Although VLC has been widely used in pharmaceutical

food and somatic industries

it was still a great challenge for new researchers in the field of separation and purification of TCM due to the lack of systematic disclosure of the technique. The characteristics

principle and key operating points of VLC and the separation strategy in laboratory were introduced in this paper to help study the separation and purification of TCM.

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Related Author

Qing-song YUAN

Tao ZHOU

Jiao XU

Wei-ke JIANG

Jin-qiang ZHANG

Cheng PAN

Yuan YUAN

Xiao WANG

Related Institution

Guizhou University of Traditional Chinese Medicine（TCM）

National Resource Center for Chinese Meteria Medica， Chinese Academy of Chinese Medical Sciences

Shandong Analysis and Test Center，Ji'nan

Hubei University of Chinese Medicine

Guizhou Wumeng Teng Fungus Industry Limited Company，Dafang

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