Objective: To establish a qualitative and quantitative method which can simultaneously measure the main components of Liangfuwan

and provide a reference for its quality control. Method: Thin layer chromatography (TLC) method was used for qualitative determination of 5 characteristic components:galangin

kaempferol

cyperotundone

nootkatone and α-cyperone. In the TLC method

silica gel GF254 was used as thin layer plate and petroleum ether (60-90℃)-ethyl acetate (5:1) was used as the developing agent; the results were viewed under the wavelength of 254 nm and 365 nm. High performance liquid chromatography (HPLC) determination method was established to simultaneously determine the contents of the above components. HPLC method was performed on Phenomenex C18 column (4.6 mm×250 mm

5 μm)

with methanol (A)-0.2%phosphoric acid aqueous solution (B) as mobile phase for gradient elution (0-30 min

55%A; 30-45 min

55%-70%A; 45-70 min

70% A) at a flow of 1.0 mL·min-1. The detection wavelength was 242 nm and column temperature was 30℃. Result: The method could be used to simultaneously determine five components in Liangfuwan under the same condition

and the mass fraction was 3.33-3.81

1.13-1.29

0.57-1.12

0.02-0.09

0.36-0.54 mg·g-1 respectively for galangin

kaempferol

cyperotundone

nootkatone and α-cyperone. Conclusion: This method is simple

feasible and reproducible

which can provide a good reference for further improvement of quality control of Liangfuwan.