LI Yan, ZHAO Meng-jie, YUAN An, et al. Effect of Dosage on Pharmacokinetic Characteristics of Total Alkaloids from Aconiti Lateralis Radix Praeparata in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(22): 82-85.
DOI:
LI Yan, ZHAO Meng-jie, YUAN An, et al. Effect of Dosage on Pharmacokinetic Characteristics of Total Alkaloids from Aconiti Lateralis Radix Praeparata in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(22): 82-85. DOI: 10.13422/j.cnki.syfjx.2016220082.
Effect of Dosage on Pharmacokinetic Characteristics of Total Alkaloids from Aconiti Lateralis Radix Praeparata in Rats
Objective: To explore pharmacokinetic characteristics of total alkaloids from Aconiti Lateralis Radix Praeparata with different dosage in rats
and discuss the relationship between the pharmacokinetics and the dosage. Method: Rats were randomly divided into the low
medium and high dosage groups by oral administration of total alkaloids from Aconiti Lateralis Radix Praeparata with the dosage of 9.6
19.2
38.4 mg·kg-1
respectively.A series of blood samples were collected through orbital venae angularis at time points of 0
5
10
20
30
40
60
120
180
300
420
540
720
1 440 min into heparinized tubes.The plasma samples were centrifuged and separated.UHPLC-Q-TOF-MS was established to detect blood drug concentrations of aconitine
mesaconitine
hypaconitine
benzoylaconitine
benzoylmesaconine
benzoylhypacoitine and aconine at different time points.Several concentration-time curves were drawn and pharmacokinetic parameters were calculated. Result: Compared with the low dosage group
Cmax and AUC0-∞ of seven alkaloids in the middle and high dosage groups increased
most of them had significant difference;the majority of Vz/F
CL/F
t1/2 and Tmax did not have significant difference. Conclusion: The dosage has a significant impact on pharmacokinetic characteristics of total alkaloids from Aconiti Lateralis Radix Praeparata in rats.In the range of low dosage to high dosage
the process in vivo of total alkaloids is basically in line with the linear dynamic characteristics.
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Related Author
JIANG Li-yun
HUANG Yu-mei
WU Zhi-jian
MAI Min-xin
GAO Ming
WEN Jin-qing
YE Zhi-long
MA En-yao
Related Institution
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine
School of Chinese Materia Medica, Beijing University of Chinese Medicine
College of Basic Medicine and Forensic Medicine, Henan University of Science and Technology
Beijing University of Chinese Medicine
The First Affiliated Hospital of Hunan University of Chinese Medicine