Objective: To observe the effect from total flavonoids of Oxytropis Falcate Bunge on blood glucose and insulin resistance in type 2 diabetic KKAy mice. Method: Totally 40 SPF level male KKAy mice were randomly divided into model group

total flavonoids from Oxytropis Falcate Bunge low

medium and high dose groups

and positive control group

n=8 in each group. Another eight C57BL/6J mice were used as normal group. The mice in normal group and model group were given with normal saline; total flavonoids from Oxytropis Falcate Bunge low

medium and high dose groups respectively received 100

200

400 mg·kg-1·d-1 dose administration by ig

and the positive control group received 10 mg·kg-1·d-1 dose of pioglitazone. The treatment was four weeks for all the groups. The body weight

fasting plasma glucose and postprandial glucose were monitored weekly. After four weeks

orbital venous blood was taken and serum was taken centrifugally; pancreas tissues were taken. Fasting insulin levels were tested by ELISA assay

and insulin secretion index (ISI) was calculated; islet structure changes were observed under light microscope after HE staining of pancreatic tissues. Result: As compared with the normal group

fasting plasma glucose was significantly increased in model group mice

and 2 h plasma glucose was significantly increased; insulin level was significantly increased

and the insulin sensitivity index was significantly decreased (P<0.05)

with more obvious pancreatic tissue lesions. Total flavonoids from Oxytropis Falcate Bunge had little effect on reducing body weight in diabetic mice. As compared with the model group

at ig 7 d and 28 d

fasting plasma glucose level was significantly reduced in total flavonoids from Oxytropis Falcate Bunge groups; at ig 14 d

the 2 h plasma glucose level was significantly reduced (P<0.05). After treatment for 28 d

as compared with the model group

the total flavonoids from Oxytropis Falcate Bunge high dose group could promote insulin secretion; middle dose group could enhance the insulin sensitivity (P<0.05); HE staining showed that each dose group could protect islet β cells and delay pancreatic failure. Conclusion: Total flavonoids from Oxytropis Falcate Bunge could reduce blood glucose

promote insulin secretion

and improve insulin resistance to a certain extent in type 2 diabetic KKAy mice.