Objective: To explore the effect of Gehua Jiejiu Dizhi decoction on the liver pregnane X receptor (PXR)

constitutive androstane receptor (CAR) and their target genes CYP3A11

CYP3A25

CYP2B9 and CYP2B10 in mice with alcoholic fatty liver. Method: Twenty-nine C57BL/6J mice were randomly divided into four groups

namely normal control group

model group

and Gehua Jiejiu Dizhi decoction high-dose and low-dose groups. At first

the alcoholic fatty liver model in mice was prepared by using National Institute on Alcohol Abuse and Alcoholism (NIAAA) method. Then

Gehua Jiejiu Dizhi decoction was given orally at doses of 4.9

2.45 g·kg-1·d-1 by gavage (once a day for up to 9 days). Serum and liver tissue samples were collected for biochemical and histopathological examination of liver injury. Enzyme linked immunosorbent assay(ELISA) was performed to detect free cholesterol(FCHOL) and triglyceride(TG) levels. HE staining was used to detect liver injury. Real-time PCR and Western blot were used to examine the expressions of PXR

CAR and their target genes CYP3A11

CYP3A25

CYP2B9 and CYP2B10 in mice liver. Result: Compared with model group

hepatic steatosis significantly reduced in Gehua Jiejiu Dizhi decoction-treated mice in a dose-dependent manner. Compared with model group and blank group

expressions of PXR and CYP3A25 significantly increased in Gehua Jiejiu Dizhi decoction-treated mice (P<0.01). CAR mRNA level significantly rose (P<0.05). Compared with normal group

FCHOL and TG levels significantly increased in model group (P<0.01). Conclusion: Gehua Jiejiu Dizhi decoction could significantly ameliorate AFLD in mice

which was possibly correlated with the activation of PXR and its regulatory genes CYP3A25.