Effect of Salviae Miltiorrhizae Radix et Rhizoma in Shenxiong Glucose Injectionon Cytochrome P450 in Mice

WANG Wen-hua; YANG Yang; WU Qiong; ZHENG Lin; LI Li; HE Bin; WANG Ai-min; LI Yong-jun; LIU Ting

doi:10.13422/j.cnki.syfjx.2016220150

您当前的位置：

首页 >

文章列表页 >

Effect of Salviae Miltiorrhizae Radix et Rhizoma in Shenxiong Glucose Injectionon Cytochrome P450 in Mice

更新时间：2024-01-04

- Effect of Salviae Miltiorrhizae Radix et Rhizoma in Shenxiong Glucose Injectionon Cytochrome P450 in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 22, Pages: 150-153(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016220150
  CLC： R285
- Published：2016
- 稿件说明：
移动端阅览
WANG Wen-hua, YANG Yang, WU Qiong, et al. Effect of Salviae Miltiorrhizae Radix et Rhizoma in Shenxiong Glucose Injectionon Cytochrome P450 in Mice[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(22): 150-153.
DOI：

WANG Wen-hua, YANG Yang, WU Qiong, et al. Effect of Salviae Miltiorrhizae Radix et Rhizoma in Shenxiong Glucose Injectionon Cytochrome P450 in Mice[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(22): 150-153. DOI： 10.13422/j.cnki.syfjx.2016220150.

摘要

目的：考察参芎葡萄糖注射液中的主要组分丹参（Salviae Miltiorrhizae Radix et Rhizoma，SM）水提取物对小鼠细胞色素P450酶（CYPs）主要亚型（CYP1A2，CYP2D22，CYP3A11和CYP2E1）的影响。方法：昆明种雄性小鼠分为5组，分别为正常组，苯巴比妥组，丹参水提取物组（3.9 mg·kg-1·d-1），丹参低、高剂量组（2.6，5.2 mg·kg-1·d-1），连续ig 7 d给药。取各组小鼠的肝脏组织，提取肝脏中RNA以及制备肝微粒体，检测各组小鼠CYP450酶活性，mRNA和蛋白水平的变化。结果：与正常组比较，丹参高剂量组可以显著降低CYP2E1的酶活性，mRNA和蛋白表达的水平（P<0.05），丹参对CYP1A2，CYP3A11，CYP2D22没有显著性影响。结论：参芎葡萄糖注射液中的丹参组分可以影响CYP2E1的酶活性，mRNA以及蛋白的表达。

Abstract

Objective: To investigate the effect of water extracts from the main component Salviae Miltiorrhizae Radix et Rhizoma (SM) in Shenxiong glucose injection on subtypes (CYP1A2

CYP2D22

CYP3A11 and CYP2E1) of mice cytochrome P450(CYP450). Method: Malemice from Kunming were randomly divided into blank group

phenobarbital group

and SM water extract group (3.9 mg·kg-1·d-1)

and SM low and high-dose groups (2.6

5.2 mg·kg-1·d-1). Then the mice were killed after the animals were administered with medicines by gavageonce daily for consecutively 7 days. Liver RNAs were collected to prepare microsomes

in order to determine the enzyme activities of CYP2E1

CYP2D22

CYP1A2 and CYP3A

and quantitative real-time PCR and Western blot was employed to examine the mRNA and protein expressions of these four CYP450 enzymes in liver tissues of mice. Result: Compared with the normal group

CYP2E1 activity

and mRNA and protein expressions were decreased in the SM high-dose group (P<0.05). SM had no effect on the activities of CYP1A2

CYP3A and CYP2D22. Conclusion: SM in Shenxiong glucose injection has effects on CYP2E1 enzymatic activity

and mRNA and protein expressions.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Slicing Angle and Initial Water Content on Water Migration and Effective Ingredient Content in Drying Process of Salviae Miltiorrhizae Radix et Rhizoma

Pharmacological Effects and Mechanisms of Salviae Miltiorrhizae Radix et Rhizoma and Its Active Components in Treating Depression： A Review

Active Components of Salviae Miltiorrhizae Radix et Rhizoma and Its Compound in Treatment of Nervous System Diseases： A Review

Molecular Mechanism of Salviae Miltiorrhizae Radix et Rhizoma against Bladder Cancer： Based on Network Pharmacology and In Vitro Experiment

New Progress in Mechanism of Salviae Miltiorrhizae Radix et Rhizoma in Treatment of Diabetes and Its Complications

Related Author

YANG Guohong

ZHOU Bingqian

LU Heng

WANG Xiao

GUO Lanping

LIU Wei

HUANG Ziyang

WANG Zhiyi

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Pharmacy，Shandong Analysis and Test Center，Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province，Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province， Qilu University of Technology（Shandong Academy of Sciences）

Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao，Academy of Chinese Medical Sciences，Henan University of Chinese Medicine

Institute of Traditional Chinese Medicine， Heilongjiang University of Chinese Medicine

Graduate School，Heilongjiang University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰