Objective: To establish a method for the determination of entrapment efficiency of glucose-modified paclitaxel-loaded brain targeting liposomes. Method: The liposomes were prepared by film dispersion method.The content of paclitaxel was detected by RP-HPLC with mobile phase of methanol-water (70:30)

detection wavelength at 228 nm and flow rate of 0.8 mL·min-1.The content of cholesterol was determined by RP-HPLC with mobile phase of methanol

detection wavelength at 210 nm and flow rate of 1.0 mL·min-1.The separation of free paclitaxel and liposomes was performed with gel column chromatography

protamine precipitation

filter membrane method and dialysis method to compare their feasibility for determination of entrapment efficiency. Result: The specificity

RSD of precision

stability and recovery test of both chromatographic methods for paclitaxel and cholesterol all met the demand of determination.Linear ranges of paclitaxel and cholesterol were 0.4-100

1-500 mg·L-1

respectively.Recoveries of paclitaxel and liposomes from four methods were all 95%-105%

which met the requirement of determination of entrapment efficiency.Entrapment efficiencies in gel column chromatography

protamine precipitation

filter membrane method and dialysis method were 74.68%

92.91%

95.14% and 95.08%. Conclusion: Protamine precipitation method is hardly affected by particle size of the liposomes

it is suitable for determination of entrapment efficiency of paclitaxel liposomes.