YANG Li-fang, LIU Hong-cun, ZHI Yuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Based on Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 28-34.
DOI:
YANG Li-fang, LIU Hong-cun, ZHI Yuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Based on Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 28-34. DOI: 10.13422/j.cnki.syfjx.2016230028.
Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Based on Response Surface Method
Objective: To optimize the extraction conditions of total alkaloids from Derris eriocarpa by response surface method
and detect their antibacterial activity. Method: Microwave extraction was used for one-single factor experiment of D. eriocarpa to investigate the effects of solid-liquid ratio
ethanol volume fraction
extraction time
microwave power
and number of extraction times on the extraction of total alkaloids from D. eriocarpa. Response surface method (RSM) was employed to optimize the extraction process
improve the extraction efficiency of total alkaloids
and investigate the in vitro antibacterial effect of total alkaloids. Result: The optimal extraction parameters were as follows:microwave extraction power of 400 W
extraction temperature of 70℃
solid-liquid ratio of 1:23
extraction time of 23 min and ethanol volume fraction of 70%. The experiment extraction yield of the total alkaloids was 0.107 7 mg·g-1 under these optimal conditions measured by berberine hydrochloride. The antibacterial activity data suggested that the total alkaloids had a certain inhibitory effect on the Staphylococcus aureus. Conclusion: The microwave extraction method for total alkaloids from D. eriocarpa was efficient and stable
and the total alkaloids showed good antibacterial effect.
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