Screening for Endophytic Fungi of  Producing Nepodin-8--Glucopyranoside and Polydatin

DING Chang-hong; GUO Mei; WANG Qian-bo; WANG Zhen-yue

doi:10.13422/j.cnki.syfjx.2016230045

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Screening for Endophytic Fungi of Producing Nepodin-8--Glucopyranoside and Polydatin

更新时间：2024-01-04

- Screening for Endophytic Fungi of Producing Nepodin-8--Glucopyranoside and Polydatin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 23, Pages: 45-48(2016)
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- DOI：10.13422/j.cnki.syfjx.2016230045
  CLC： Q93
- Published：2016
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DING Chang-hong, GUO Mei, WANG Qian-bo, et al. Screening for Endophytic Fungi of Producing Nepodin-8--Glucopyranoside and Polydatin[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 45-48.
DOI：

DING Chang-hong, GUO Mei, WANG Qian-bo, et al. Screening for Endophytic Fungi of Producing Nepodin-8--Glucopyranoside and Polydatin[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 45-48. DOI： 10.13422/j.cnki.syfjx.2016230045.

摘要

目的：在前期的研究工作中已从不同产地分离得到毛脉酸模内生真菌278株，以这些内生真菌为研究对象，寻找能够通过自身发酵产生酸模素苷、白藜芦醇苷的内生真菌。方法：通过HPLC检测内生真菌的次生代谢产物，以酸模素苷、白藜芦醇苷为参照，筛选能够产生酸模素苷、白藜芦醇苷的内生真菌，并测定其含量。结果：发现其中的11株内生真菌的次生代谢产物中含有酸模素苷，5株次生代谢产物中含有白藜芦醇苷，其中内生真菌tq-s1的次生代谢产物中酸模素苷的质量分数最高（530 μg·g-1），且白藜芦醇苷的质量分数也最高（224 μg·g-1）。结论：内生真菌的种类和其次生代谢产物的形成受到地理位置的影响，对于酸模素苷和白藜芦醇苷的高产菌种tq-s1，可以进行开发利用的研究。

Abstract

Objective: In the previous study

278 strains of endophytic fungi were separated from Rumex gmelini in different origins. These endophytic fungi were taken as the research object to screen out endophytic fungi that could produce nepodin-8-O-β-D-glucopyranoside and polydatin through fermentation. Method: HPLC was used to detect secondary metabolites of endophytic fungi

nepodin-8-O-β-D-glucopyranoside and polydatin were used as reference to screen out endophytic fungi that could produce nepodin-8-O-β-D-glucopyranoside and polydatin

and determine their content. Result: Eleven strains of endophytic fungi whose secondary metabolites containing nepodin-8-O-β-D-glucopyranoside were found

meanwhile 5 strains could produce polydatin. Among them

fungi tq-s1 had the highest mass fraction of nepodin-8-O-β-D-glucopyranoside (530 μg·g-1) and polydatin (224 μg·g-1) in its secondary metabolites. Conclusion: Endogenous fungal species and formation of their secondary metabolites were affected by geographical location. Further studies shall be conducted on the development and utilization of high-yield strain tq-s1 of both nepodin-8-O-β-D-glucopyranoside and polydatin.

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