GAO Xiu-rong, XU Xiao-hong, YANG Yang, et al. Pharmacokinetic Analysis of Tetrandrine Sustained-release Thermosensitive Gel in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 98-102.
DOI:
GAO Xiu-rong, XU Xiao-hong, YANG Yang, et al. Pharmacokinetic Analysis of Tetrandrine Sustained-release Thermosensitive Gel in Rats[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 98-102. DOI: 10.13422/j.cnki.syfjx.2016230098.
Pharmacokinetic Analysis of Tetrandrine Sustained-release Thermosensitive Gel in Rats
Objective: LC-MS/MS was employed to determine blood concentration after intravenous
intragastric administration of tetrandrine powder and subcutaneous injection of tetrandrine gel
and calculated the bioavailability of tetrandrine gel. Method: Determination of tetrandrine in plasma was established.After intravenous
intragastric administration of tetrandrine powder and subcutaneous injection of tetrandrine gel
LC-MS/MS was adopted to determine blood concentration of tetrandrine
detection ion for tetrandrine was m/z 623.1-176.0
detection ion for dauricine (the internal standard) was m/z 625.1-206.0
DAS 3.0 software was used to calculate pharmacokinetic parameters and the absolute bioavailability. Result: This established LC-MS/MS had good stability and precision
extraction recovery was 81.36%-97.14%.Absolute bioavailabilities of intragastric administration of tetrandrine powder and subcutaneous injection of tetrandrine gel were 42.76% and 85.44%
respectively. Conclusion: The absolute bioavailability of tetrandrine gel is improved greatly by compared with intragastric administration of tetrandrine powder
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