Objective: To study the effect of Huangjin capsule on protein expressions of phosphatidylinositol-3-kinasem(PI-3K)

glucose transporter 4(GLUT4) in liver and skeletal muscle tissue of diabetes mellitus and insulin resistance rats. Method: Totally 65 SPF male Wistar rats with the blood glucose ranging between 4.77-7.77 mmol·L-1.Except the 12 rats in normal group

all of the remaining rats were given high-fat diet and 1%streptozotocin 50 mg·kg-1 to build the rat model of diabetes mellitus. The diabetic rats were randomly divided into Huangjin capsule group(Huangjin capsule suspension

2.025 g·kg-1)

rosiglitazone (rosiglitazone suspension

0.36 mg·kg-1). Model group was continuously fed with high-fat diet

andtreated by the corresponding drugs together with normal group for 10 weeks. Both model group and normal group were given normal saline. The general state of the rats

body weight and food intake were observed. 10 weeks later after the intervention

10%chloral hydrate was injected to anesthetize the rats totest fasting blood-glucose(FBG)

fasting insulin(FINS)

and insulin sensitivity index(ISI). The immunochemical method was used to detect the pathological changes in rat liver and skeletal muscle tissue

and HE was applied to detect PI-3K and GLUT4 protein expressions. Result: Compared with normal group

model group showed significant increases in FBG

FINS levels

and decreases in ISI

PI-3K and GLUT4 protein expressions in rat liver and skeletal muscle tissue (P<0.05)

and the pathological detection showed obvious lesions in rat liver and skeletal tissue.After drug intervention

compared with model group

both Huangjin capsule group and rosiglitazone group can reduce the level of FBG and FINS of diabetes mellitus and insulin resistance rats

increase the level of ISI

PI-3K and GLUT4 protein expressions in rat liver and skeletal muscle tissue(P<0.05)

with obvious alleviation in lesions in rat liver and skeletal tissue. Conclusion: Huangjin capsule has the effect in improving insulin sensitivity

the mechanism is correlated with the activation of PI-3K and GLUT4 protein expression in insulin signal pathway of skeletal muscle tissue.