JIANG Xu-guang, WANG Cheng-wen, LIANG Xin-jing, et al. Protective Effect of Shenling Baizhu Granule on Ulcerative Colitis Model[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 144-148.
DOI:
JIANG Xu-guang, WANG Cheng-wen, LIANG Xin-jing, et al. Protective Effect of Shenling Baizhu Granule on Ulcerative Colitis Model[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 144-148. DOI: 10.13422/j.cnki.syfjx.2016230144.
Protective Effect of Shenling Baizhu Granule on Ulcerative Colitis Model
Objective: To study the protective effect and mechanism of Shenling Baizhu granule (SLBZ) on ulcerative colitis in mice induced by dextran sulfate sodium (DSS). Method: Clean-grade Balb/c mice were randomly divided into control group
3 d model group
3 d SLBZ group
7 d model group and 7 d SLBZ group. 3 d and 7 d SLBZ groups were given SLBZ (9 g·kg-1·d-1) by gavage for 8 days
while control group and two model groups were given the same volume of saline. In all groups except the control group
animal models were established with 4% DSS water in the last 3 days. 3 d model group and 3 d SLBZ group were observed and tested on 3 d after modeling
while control group
7 d model group and 7 d SLBZ group were observed and tested on 7 d after modeling. The changes in body weight
stool index (diarrhea and bloody stool)
pathological score
apoptosis index
mucus expression and occludin protein were observed. Result: Compared with the normal group
the amount of apoptotic epithelial cells in colonic mucosa of 3 d model group increased significantly
the expression of occludin was significantly reduced (P<0.05). In 7 d model group
body weight decreased significantly
stool index increased significantly
the pathological score increased
the amount of apoptotic cells increased
and occludin protein expression was significantly reduced (P<0.05). Compared with the model group
after preventive gavage of SLBZ suspension for 8 days
the amount of apoptotic epithelial cells in 3 d SLBZ group decreased
occludin protein expression increased significantly (P<0.05)
body weight of mice in 7 d SLBZ group increased
diarrhea and bloody stool symptoms improved obviously
pathological score decreased
the amount of mucus secretion increased
apoptotic cells decreased
and the expression of occludin increased (P<0.05). Conclusion: The preventive administration with SLBZ could relieve the symptoms of colitis induced by DSS
which may be closely related to protection of SLBZ on colonic mucosal barrier.
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