ZHANG Yu, YANG Jian, QIN Zhen-xian, et al. Optimization of Separation and Enrichment Process of Iridoid Glycosides from Lonicerae Japonicae Flos by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 18-21.
DOI:
ZHANG Yu, YANG Jian, QIN Zhen-xian, et al. Optimization of Separation and Enrichment Process of Iridoid Glycosides from Lonicerae Japonicae Flos by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 18-21. DOI: 10.13422/j.cnki.syfjx.2016240018.
Optimization of Separation and Enrichment Process of Iridoid Glycosides from Lonicerae Japonicae Flos by Macroporous Resin
Objective: To optimize separation and purification technology of iridoid glycosides from Lonicerae Japonicae Flos by macroporous resin. Method: The content of loganic acid
7-epi-loganin
sweroside and secoxyloganin was determined by UPLC
static adsorption-elution test was adopted to screen type of macroporous resin
separation and purification process conditions of iridoid glycoside were optimized by single factor tests. Result: H-103 macroporous resin was selected
optimum technology was:sample solution concentration of 100 g·L-1
adsorption flow rate of 2.0 BV·h-1
sample liquid volume of 2.5 BV
eluted by 2.5 BV of water and 3.0 BV of 30% ethanol at elution flow rate of 2.0 BV·h-1.Under these conditions
elution rate and purity of iridoid glycosides were 90.0% and 25.6%
respectively. Conclusion: Iridoid glycosides from Lonicerae Japonicae Flos are purified effectively with H-103 macroporous resin
Optimization of Purification Technology for Evodiamine from Euodiae Fructus by Macroporous Resin
Comparison of Enrichment Effect of Iridoid Glycosides from by Different Macroporous Resins
Anti-inflammatory Effect and Mechanism of Active Constituents from Lonicerae Japonicae Flos and Lonicerae Flos: A Review
Optimization of Extraction and Purification Process of Total Iridoids and Total Crocins in Gardeniae Fructus
Analysis of Chemical Composition Variations in Plantaginis Semen at Different Frying Time Based on UPLC-Q-TOF/MSE
Related Author
LI Shu-ying
LI Xiao-fei
CHEN Fei
SUN Hong-sheng
CAI Li-ming
ZHOU Ying
CAI Bao-chang
LIU Fang
Related Institution
Basic Research Center of Integrated Chinese and Western Medicine for Prevention and Treatment of Vascular Diseases,Hunan University of Chinese Medicine
Key Laboratory of Vascular Biology and Translational Medicine,Hunan University of Chinese Medicine
School of Pharmacy,Tianjin University of Traditional Chinese Medicine
School of Pharmacy, Jiangxi University of Chinese Medicine
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences