Identification of Steroidal Saponins in Ophiopogonis Radix by UPLC-Q-TOF-MS Combined with Relative Retention Time

YAN Ren-yi; MA Feng-xia; YU He-shui; KANG Li-ping; ZHANG Jie; ZHAO Yang; SUN Xin-guang; JIA De-xian; MA Bai-ping

doi:10.13422/j.cnki.syfjx.2016240043

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Identification of Steroidal Saponins in Ophiopogonis Radix by UPLC-Q-TOF-MS Combined with Relative Retention Time

更新时间：2024-01-04

- Identification of Steroidal Saponins in Ophiopogonis Radix by UPLC-Q-TOF-MS Combined with Relative Retention Time
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 24, Pages: 43-50(2016)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2016240043
  CLC： R284.1
- Published：2016
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YAN Ren-yi, MA Feng-xia, YU He-shui, et al. Identification of Steroidal Saponins in Ophiopogonis Radix by UPLC-Q-TOF-MS Combined with Relative Retention Time[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 43-50.
DOI：

YAN Ren-yi, MA Feng-xia, YU He-shui, et al. Identification of Steroidal Saponins in Ophiopogonis Radix by UPLC-Q-TOF-MS Combined with Relative Retention Time[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 43-50. DOI： 10.13422/j.cnki.syfjx.2016240043.

摘要

目的：采用超高效液相色谱-飞行时间质谱（UPLC-Q-TOF-MSE）表征并快速在线鉴定麦冬中甾体皂苷类成分；结合呋甾皂苷在反向色谱上的保留规律，鉴别同分异构体。方法：运用2种反相色谱柱（ACQUITY UPLC HSS T3和Agela Venusil XBP C18-2），在2种溶剂系统（0.1%甲酸/水-0.1%甲酸/乙腈，丙酮-水）和3个洗脱条件下对系列呋甾皂苷的色谱保留规律进行总结。利用UPLC-Q-TOF-MSE对麦冬成分进行分析，获得质谱数据。结果： 3种色谱条件下呋甾皂苷的相对保留时间一致，呈现如下保留规律：甾体皂苷中糖链结构相同，苷元上羟基的数目越多保留时间越短；仅羟基位置不同时，保留时间为1位羟基< 17位羟基< 14位羟基；仅C-26位糖链糖基连接位置不同时，葡萄糖-（1→6）-葡萄糖结论：色谱保留时间能为呋甾皂苷同分异构体的液质联用在线鉴定提供有益的数据支持。

Abstract

Objective: To identify the steroidalsaponins in the tubers of Ophiopogonis Radix by ultra-performance liquid chromatography tandem with time-of-fight mass spectrometry (UPLC-Q-TOF-MSE)combined with the relative retention time (RRT) of furostanolsaponins in reverse chromatography. Method: The retention time of several furostanolsaponins was tested on two kinds of reversed-phase column

Acquity UPLC HSS T3 and Agelavenusil XBP C18-2

using water (0.1% formic acid)-acetonitrile (0.1% formic acid) and acetone-water

respectively. The mass spectrometric data of Ophiopogonis Radix were obtained by UPLC-Q-TOF-MSE. Result: The RRT of furostanolsaponins was consistently in three chromatographic conditions. When the sugar parts were identical

the RRT was shorter alone with the increase number of hydroxyl; location of hydroxyl affected the RRT

the orders were asfollow:1-hydroxyl< 17-hydroxyl< 14 -hydroxyl. When only the sugar chain at C-26 was different

the RRT was as follow:glucosyl-(1→6)-glucosylEcombined with RRT

a total of 21 compounds were identifiedincluding 14 furostanolsaponins and 7 spirostanolsaponins. Conclusion: The RRT of furostanolsaponins provide supportive data for rapidly identify isomers of steroidalsaponins from Ophiopogonis Radix by UPLC-Q-TOF-MSE.

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