WANG Yu-rong, XING Shao-fang, LI Zhong-zhu, et al. Antioxidant and Antimicrobial Activities of Components from [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 59-63.
DOI:
WANG Yu-rong, XING Shao-fang, LI Zhong-zhu, et al. Antioxidant and Antimicrobial Activities of Components from [J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 59-63. DOI: 10.13422/j.cnki.syfjx.2016240059.
Antioxidant and Antimicrobial Activities of Components from
Objective: To obtain the information about the antioxidant and antimicrobial activities of components from the fresh flower bud of Zingiber mioga. Method: The flower bud of Z. mioga was extracted and concentrated with methanol
and after extraction with petroleum ether
dichloromethane
ethyl acetate
n-butanol and water
the five fractions were evaluated for antioxidant activities by DPPH radical scavenging method and antibacterial activities on four strains (Escherichia coli
Salmonella lignieres
Pseudomonas aeruginosa and Staphylococcus aureus) by agar disc diffusion method. Result: The extraction rate of Z. mioga flower bud was 3.1% in heating reflux method; the ethyl acetate extract showed the highest scavenging capacity on DPPH radical with IC50 of (26.22±1.12) mg·L-1
and its inhibition zone on Pseudomonas aeruginosa was (10±1) mm; petroleum ether fraction showed highest antibacterial activities
with minimal inhibitory concentration(MIC) of 25.0 mg·L-1 on Staphylococcus aureus. Conclusion: All of fractions of the methanol extract from Z. mioga flower bud showed antioxidant abilities. Their potency of antibacterial activity was as follows:petroleum ether fraction > dichloromethane fraction > ethyl acetate fraction > methanol extract
providing theoretical basis for the utilization and development of Z. mioga flower bud antioxidant and bacteriostatic products.