Correlation Between Effective Components and Powder Color of Celosiae Cristatae Flos

GUO Shuang; LI Qing; HE Wan-wan; KANG Ting-guo

doi:10.13422/j.cnki.syfjx.2016240064

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Correlation Between Effective Components and Powder Color of Celosiae Cristatae Flos

更新时间：2024-01-04

- Correlation Between Effective Components and Powder Color of Celosiae Cristatae Flos
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 24, Pages: 64-69(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016240064
  CLC： R284.1
- Published：2016
- 稿件说明：
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GUO Shuang, LI Qing, HE Wan-wan, et al. Correlation Between Effective Components and Powder Color of Celosiae Cristatae Flos[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 64-69.
DOI：

GUO Shuang, LI Qing, HE Wan-wan, et al. Correlation Between Effective Components and Powder Color of Celosiae Cristatae Flos[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 64-69. DOI： 10.13422/j.cnki.syfjx.2016240064.

摘要

目的：研究鸡冠花药材粉末颜色与其有效成分含量的相关性。方法：用电子感观分析法（色度计）测量药材粉末L*，a*，b*值，用RP-HPLC法同时测定鸡冠花4种有效成分含量，用紫外-可见分光光度法测定鸡冠花总黄酮（以芦丁计）含量，用SPSS 19.0分析颜色值与有效成分含量间的相关性。结果： L*与木犀草素、槲皮素、山柰酚、异鼠李素含量呈极显著负相关；a*与木犀草素、槲皮素、山柰酚含量呈极显著正相关；b*与木犀草素、槲皮素、山柰酚呈极显著负相关。a*，b*与异鼠李素含量不相关，L*，a*，b*与总黄酮（以芦丁计）的含量不相关。结论：鸡冠花粉末颜色越暗、偏红程度越大，其木犀草素、槲皮素、山柰酚含量越高，而偏黄程度大，则木犀草素、槲皮素、山柰酚含量越低；鸡冠花异鼠李素含量仅与鸡冠花粉末明暗程度相关（负相关）；鸡冠花总黄酮含量（以芦丁计）与其粉末颜色不相关。木犀草素、槲皮素、山柰酚代表了植物中最多的黄酮醇类化合物，因此用它们作对照品来分析药材有效成分与粉末颜色相关性有一定意义，且测定鸡冠花的颜色值用以评价其质量具有简便、节能的优点，可为鸡冠花的质量控制提供新的途径和参考。另外，鸡冠花主要色素类成分为红色素，鸡冠花粉末颜色值是否反应鸡冠花红色素的含量以及鸡冠花具体发挥作用的成分有待于进一步研究。

Abstract

Objective: To study the correlation between the color of Celosiae Cristatae Flos powder and its chemical components. Method: Colorimeter was used to determined L*

a* and b* values of Celosiae Cristatae Flos powder

RP-HPLC was used to determine the content of the four active components

spectrophotometer was adopted to determined total flavonoids in Celosiae Cristatae Flos (based on rutin)

and SPSS 19.0 software was used to analyze the correlation between color values and content of active component in this study. Result: In Celosiae Cristatae Flos

L* and luteolin

quercetin

kaempferol

isorhamnetin were significantly negatively correlated; a* and luteolin

quercetin

kaempferol were significantly positively correlated; and b* and luteolin

quercetin

kaempferol were significantly negatively correlated. a*

b* and isorhamnetin were uncorrelated

and L*

b* and total flavonoids (based on rutin) were significantly negatively correlated. Conclusion: There was a significant correlation between the color values and the content of the four effective components. It is energy-saving and convenient to assess the quality control of Celosiae Cristatae Flos by determining the color of powder. Although the new method can be applied to assess the quality of Celosiae Cristatae Flos and provide reference

some problems still shall be further studied.

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