Pharmacokinetics of Three Alkaloids from Shiwuwei Rupeng Pills in Rats by UPLC-MS/MS

ZHANG Wu-gang; ZHAN Yuan; FENG Yu-lin; YANG Jie; CHEN Hai-fang; YANG Shi-lin

doi:10.13422/j.cnki.syfjx.2016240095

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Pharmacokinetics of Three Alkaloids from Shiwuwei Rupeng Pills in Rats by UPLC-MS/MS

更新时间：2024-01-04

- Pharmacokinetics of Three Alkaloids from Shiwuwei Rupeng Pills in Rats by UPLC-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 24, Pages: 95-98(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016240095
  CLC： R285.5
- Published：2016
- 稿件说明：
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ZHANG Wu-gang, ZHAN Yuan, FENG Yu-lin, et al. Pharmacokinetics of Three Alkaloids from Shiwuwei Rupeng Pills in Rats by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 95-98.
DOI：

ZHANG Wu-gang, ZHAN Yuan, FENG Yu-lin, et al. Pharmacokinetics of Three Alkaloids from Shiwuwei Rupeng Pills in Rats by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 95-98. DOI： 10.13422/j.cnki.syfjx.2016240095.

摘要

目的：采用超高效液相色谱质谱联用法测定大鼠灌胃十五味乳鹏丸后体内3种生物碱（乌头碱、新乌头碱、次乌头碱）的血浆药物浓度并进行药代动力学参数评价。方法： Welch UPLC XB-C18色谱柱（2.0 mm×50 mm，1.7 μm），乙腈-0.1%甲酸水溶液梯度洗脱，流速0.35 mL·min-1，柱温40℃，进样量5 μL，采用电喷雾正离子化质谱法监测血浆中各成分的浓度。结果：乌头碱、新乌头碱和次乌头碱的线性范围分别为分别在126.56~10 125，18.75~1 500，16.32~1 305.5 ng#183;L-1。3种生物碱在大鼠体内的主要药代动力学参数药峰浓度（Cmax）分别为（0.75±0.14），（0.23±0.03），（0.17±0.02）μg·L-1，消除半衰期（t1/2）分别为（2.93±1.72），（3.02±1.60），（3.70±2.63）h，药时曲线下面积AUC0-t分别为（5.03±1.37），（1.51±0.26），（1.14±0.21）h·μg·L-1。结论：该方法快速、准确、灵敏，可用于该类成分的血药浓度测定及其药代动力学研究，为十五味乳鹏丸的体内研究提供参考。

Abstract

Objective: The concents of aconitine

mesaconitine and hypaconitine in rat plasma were determined by UPLC-MS/MS after oral administration of Shiwuwei Rupeng pills

and pharmacokinetics of these three alkaloids in rats was investigated. Method: The separation was carried out on Welch UPLC XB-C18 column (2.0 mm×50 mm

1.7 μm)

mobile phase was acetonitrile-0.1% formic acid in water for gradient elution

flow rate was 0.35 mL·min-1.Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Result: The linear ranges of aconitine

mesaconitine and hypaconitine were 126.56-10 125

18.75-1 500

16.32-1 305.5 ng·L-1

respectively.The main pharmacokinetic parameters of aconitine

mesaconitine and hypaconitine were obtained as follows:Cmax of (0.75±0.14)

(0.23±0.03)

(0.17±0.02) μg·L-1

t1/2 of (2.93±1.72)

(3.02±1.60)

(3.70±2.63) h

AUC0-t of (5.03±1.37)

(1.51±0.26)

(1.14±0.21) h·μg·L-1. Conclusion: This method is rapid

accurate and sensitive

and it can be used for pharmacokinetic study of such alkaloids

which provides pharmacokinetic evidence for in vivo study of Shiwuwei Rupeng pills.

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