Effect of Tongluo Tangtai Power on JNK Signaling Pathway in Rats of Diabetic Peripheral Neuropathy

ZHANG Xi-yu; HU Yun; XIE Chun-guang; DU Lian

doi:10.13422/j.cnki.syfjx.2016240122

您当前的位置：

首页 >

文章列表页 >

Effect of Tongluo Tangtai Power on JNK Signaling Pathway in Rats of Diabetic Peripheral Neuropathy

更新时间：2024-01-04

- Effect of Tongluo Tangtai Power on JNK Signaling Pathway in Rats of Diabetic Peripheral Neuropathy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 24, Pages: 122-127(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016240122
  CLC： R285.5
- Published：2016
- 稿件说明：
移动端阅览
ZHANG Xi-yu, HU Yun, XIE Chun-guang, et al. Effect of Tongluo Tangtai Power on JNK Signaling Pathway in Rats of Diabetic Peripheral Neuropathy[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 122-127.
DOI：

ZHANG Xi-yu, HU Yun, XIE Chun-guang, et al. Effect of Tongluo Tangtai Power on JNK Signaling Pathway in Rats of Diabetic Peripheral Neuropathy[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 122-127. DOI： 10.13422/j.cnki.syfjx.2016240122.

摘要

目的：观察通络糖泰方对糖尿病周围神经病变（DPN）大鼠JNK信号转导通路的影响，并探讨其可能的作用机制。方法： SPF级GK大鼠给予高脂饲料喂养建立DPN大鼠模型，50只造模成功的GK大鼠随机分为5组，每组大鼠均为10只，分别为模型组，通络糖泰方高、中、低剂量组，西药联合（二甲双胍+弥可保）组，同时设立正常组Wistar大鼠为10只，分别给予相应处理4周后以免疫组化方法检测坐骨神经组织磷酸化氨基末端激酶（p-JNK），半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）蛋白的表达水平，实时荧光定量聚合酶链式反应（Real-time PCR）方法检测JNK，胰岛素样生长因子1（IGF-1）mRNA的表达水平，酶联免疫吸附法检测血清肿瘤坏死因子-α（TNF-α），白细胞介素-6（IL-6），C-反应蛋白（CRP），髓磷脂碱性蛋白（MBP）水平。结果：与正常组比较，模型组p-JNK及Caspase3蛋白和JNK mRNA的表达明显升高，IGF-1 mRNA的表达明显降低，血清TNF-α，IL-6，CRP，MBP的水平明显升高（P<0.05）；与模型组比较，通络糖泰方能一定程度下调p-JNK及Caspase3蛋白和JNK mRNA的表达，上调IGF-1 mRNA的表达，明显升高坐骨神经传导速度，明显降低血清TNF-α，IL-6，CRP，MBP的水平（P<0.05）。结论：通络糖泰方可能通过抑制高糖应激诱导的JNK信号转导通路的异常激活，改善细胞凋亡、胰岛素抵抗、炎症反应、下游靶基因的异常表达等病理过程，达到防治DPN发生发展的作用。

Abstract

Objective: To investigate the effect of Tongluo Tangtai power (TLTTP) on JNK signaling pathway in rats of diabetic peripheral neuropathy (DPN). Method: Totally 70 GK rats were selected to set up the rat model of spontaneous DPN induced by high fat diet. the 50 successfully modeled DPN rats were randomly divided into five groups

namely model group

TLTTP high-dose group

TLTTP group

TLTTP Low-dose group

and metformin+methycobal group

with 10 rats in each group; another 10 male GK rats were selected in normal group. All of the rats were detected for the expressions of p-JNK and Caspase-3 protein

JNK and Insulin-like growth factor 1(IGF-1) mRNA

sciatic nerve conduction velocity

and the levels of tumor necrosis factor-α(TNF-α)

interleukins-6(IL-6)

C-reactive protein(CRP)

myelin basic protein(MBP) in serum after 4 weeks of intragastric administration. Result: Compared with the normal group

the model group showed significant increase in p-JNK and Caspase-3 proteins

JNK mRNA expression

and serum TNF-α

IL-6

CRP and MBP levels

and significant decrease in IGF-1 mRNA expression (P<0.05); TLTTP could down-regulate the expression of p-JNK

Caspase3

JNK mRNA

increase the expression of IGF-1 mRNA

significantly improve sciatic nerve conduction velocity

and significantly reduce the levels of TNF-α

IL-6

CRP

MBP in serum. Conclusion: TLTTP may prevent the development of DPN by inhibiting JNK signaling pathway

mitigating ell apoptosis

inflammation reaction and abnormal expression of downstream target genes.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Buyang Huanwutang in Treating Diabetic Peripheral Neuropathy by Inhibiting Pyroptosis Through AMPK/ULK1 Mitophagy Pathway

Inhibition of Oxidative Stress of Sciatic Nerve in Diabetic Rats by Tangbikang Granules Regulating AMPK/PGC-1α/SIRT3 Signaling Pathway

Buyang Huanwutang Treats Diabetic Peripheral Neuropathy via Mitochondrial Transport in Rats

Prevention and Treatment of Diabetic Peripheral Neuropathy by Chinese Medicine Through PI3K/Akt Signaling Pathway： A Review

Molecular Mechanism of Tangbikang Granules Against Diabetic Peripheral Neuropathy： Based on Network Pharmacology and Experimental Verification

Related Author

AN Jingwen

SONG Linchun

CHEN Die

ZHANG Wang

TIAN Jiaxin

ZHANG Tianya

BEN Ying

LIU Gang

Related Institution

College of Integrated Traditional Chinese and Western Medicine，Hebei University of Chinese Medicine，Hebei Key Laboratory of Integrated Medicine on Liver-kidney Patterns

International Joint Research Center for Diabetes Prevention and Treatment with TCM， Ministry of Science and Technology

Key Laboratory of TCM Health Sciences， Ministry of Education， Beijing University of Chinese Medicine

College of Traditional Chinese Medicine （TCM）， Beijing University of Chinese Medicine

School of Life Sciences，Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰