Effect of Zhizhu Granules on Expressions of VEGF and COX-2 in Rat Model with Chronic Atrophic Gastritis

ZHAO Fan; TANG De-cai; ZHANG Shuo; GUO Xiu-xia; YIN Gang; DAI Jian-guo

doi:10.13422/j.cnki.syfjx.2016240150

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Effect of Zhizhu Granules on Expressions of VEGF and COX-2 in Rat Model with Chronic Atrophic Gastritis

更新时间：2024-01-04

- Effect of Zhizhu Granules on Expressions of VEGF and COX-2 in Rat Model with Chronic Atrophic Gastritis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 22, Issue 24, Pages: 150-155(2016)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2016240150
  CLC： R285.5
- Published：2016
- 稿件说明：
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ZHAO Fan, TANG De-cai, ZHANG Shuo, et al. Effect of Zhizhu Granules on Expressions of VEGF and COX-2 in Rat Model with Chronic Atrophic Gastritis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 150-155.
DOI：

ZHAO Fan, TANG De-cai, ZHANG Shuo, et al. Effect of Zhizhu Granules on Expressions of VEGF and COX-2 in Rat Model with Chronic Atrophic Gastritis[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(24): 150-155. DOI： 10.13422/j.cnki.syfjx.2016240150.

摘要

目的：观察枳术颗粒对慢性萎缩性胃炎（CAG）大鼠的疗效，并探讨其作用机制。方法：选取已建立CAG模型的大鼠，分为模型组，胃复春（0.86 g·kg-1）组，枳术颗粒高、中、低剂量（20，10，5 g·kg-1）组。用蛋白免疫印迹法（Western blot），免疫组化及实时荧光定量PCR（Real-time PCR）方法检测胃组织中环氧合酶-2（COX-2），血管内皮生长因子（VEGF）蛋白及mRNA的表达。结果：与正常组比较，模型组大鼠胃黏膜腺腔结构不完整，炎细胞浸润黏膜全层，体重增长率降低，COX-2，VEGF蛋白及mRNA表达显著增加（P<0.01）；与模型组比较，枳术颗粒高、中、低剂量组及胃复春组均显著增加CAG大鼠体重增长率（P<0.01），下调大鼠胃组织COX-2，VEGF蛋白及mRNA表达（P<0.01）；与胃复春组比较，枳术颗粒高、中、低剂量组均显著增加CAG大鼠体重增长率（P<0.01），高剂量组可下调大鼠胃组织COX-2，VEGF蛋白及mRNA表达，但无明显差异。结论：枳术颗粒可加快大鼠体重增长，使CAG大鼠胃黏膜组织得到改善和恢复。其作用机制可能为下调胃黏膜破坏因子COX-2，VEGF的表达，起到预防及治疗作用。

Abstract

Objective: To observe the effect of Zhizhu granules on the rat model with chronic atrophic gastritis (CAG). Method: The rat model with chronic atrophic gastritis was established

and divided into model group

positive control group (Weifuchun

0.86 g·kg-1)

and Zhizhu granules high

medium and low-dose(20

5 g·kg-1)groups. The mRNA and protein expressions of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) were detected by Western blot

immunohistochemical (IHC) and Real-time PCR method. Result: Compared with normal group

model group showed incomplete gastric mucosa gland cavity structure

obvious inflammatory cell infiltration

significant decreasing in weight growth rate

and significant increase in COX-2 and VEGF protein and mRNA expressions (P<0.01). Compared with model group

Zhizhu granules high

medium and low-dose groups and Weifuchun group showed significant increase in CAG rats' body weight growth rate (P<0.01)

decrease in COX-2

VEGF protein and mRNA expressions in rat gastric tissues (P<0.01). Compared with Weifuchun group

Zhizhu granules high

medium and low-dose groups showed significant increase in CAG rats' body weight growth rate (P<0.01)

high-dose group could decrease the expression of COX-2

VEGF protein and mRNA expressions in rat gastric tissues

with no significant difference. Conclusion: Zhizhu granules can increase the growth of the body weight of rats

and also alleviate pathological morphology in stomach tissues. Zhizhu granules show the preventive and therapeutic effects by down-regulating the expressions of COX-2 and VEGF.

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