Correlation Between Microscopic Characteristic Index and Chemical Components of Menispermi Rhizoma

HE Wan-wan; ZHANG Jian-kui; LI Yun-jing; KANG Ting-guo

doi:10.13422/j.cnki.syfjx.2017010042

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Correlation Between Microscopic Characteristic Index and Chemical Components of Menispermi Rhizoma

更新时间：2024-01-04

- Correlation Between Microscopic Characteristic Index and Chemical Components of Menispermi Rhizoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 1, Pages: 42-46(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017010042
  CLC： R284.1
- Published：2017
- 稿件说明：
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HE Wan-wan, ZHANG Jian-kui, LI Yun-jing, et al. Correlation Between Microscopic Characteristic Index and Chemical Components of Menispermi Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 42-46.
DOI：

HE Wan-wan, ZHANG Jian-kui, LI Yun-jing, et al. Correlation Between Microscopic Characteristic Index and Chemical Components of Menispermi Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 42-46. DOI： 10.13422/j.cnki.syfjx.2017010042.

摘要

目的：研究北豆根石细胞的显微特征指数与其4种生物碱化学有效成分的相关性。方法：北豆根显微特征指数的测定采用容量分析法；4种生物碱成分青藤碱、蝙蝠葛苏林碱、蝙蝠葛碱、粉防己碱含量测定采用高效液相法；总生物碱含量测定采用紫外分光光度法，并将得到的数据进行相关性分析。结果：北豆根中石细胞的显微特征指数与其化学成分蝙蝠葛碱、蝙蝠葛苏林碱和总生物碱含量存在显著的相关性，而与青藤碱、粉防己碱则无相关性。结论：该研究初步建立了一种基于石细胞显微特征指数测定的北豆根质量评价新方法及技术，可为北豆根药材的质量控制提供新的技术手段，为其质量标准的制订提供参考。

Abstract

Objective: To study on the correlation between microscopic characteristic index of Menispermi Rhizoma and their chemical components. Method: Capacity analysis method was adopted to determine the quantify the microscopic characteristic index of Menispermi Rhizoma; high performance liquid chromatography (HPLC) method was used to determine the contents of four alkaloid components:sinomenine

daurisoline

dauricine and tetrandrine; the content of total alkaloid was determined by UV spectrophotometry. The above data were then analyzed for correlation. Result: The stone cell microscopic characteristic indexes were significantly correlated with the contents of daurisoline

dauricine and total alkaloid in Menispermi Rhizoma

but neither correlated with the content of sinomenine nor tetrandrine. Conclusion: This research established a new method and technique preliminarily based on the determination of the stone cell microscopic characteristic index

which could be applied for the quality assessment of Menispermi Rhizoma and providing references for developing its quality standard.

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Related Author

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GU Zixian

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Related Institution

School of Basic Medicine， Chengdu University of Traditional Chinese Medicine

Key Laboratory of Modern Preparation of Traditional Chinese Medicine of Ministry of Education， Jiangxi University of Chinese Medicine

Key Laboratory of Natural Medicine Pharmacology of Yunnan Province， School of Pharmacy， Kunming Medical University

School of Pharmacy， Guangxi University of Chinese Medicine

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica， Guangxi Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues， Guangxi Key Laboratory of Traditional Chinese Medicine Formulas Theory and Transformation for Damp Diseases， Guangxi University of Chinese Medicine

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