Objective: To establish the ultra performance liquid chromatography (UPLC) fingerprint method for effective quality control of Ginkgo leaves extract and Ginkgo leaves solid preparation. Method: The chromatographic fingerprint was obtained with Agilent UPLC column (2.1 mm×100 mm

1.8 μm)

with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution. The flow rate was 0.2 mL·min-1

and the column temperature was maintained at 30℃. The detection wavelength was set at 360 nm. The fingerprints of samples were further evaluated by chemometrics methods including similarity analysis(SA)

hierarchical clustering analysis(HCA) and principal component analysis(PCA). Result: The fingerprint was obtained within 16 minutes. There were 14 common peaks in the fingerprints

and 4 peaks were identified.With the fingerprint of control herb and control extract as the reference fingerprint

the samples can be divided into two types according to the similarity results:one type with similarity of 0.312-0.585

and the other type with similarity of 0.978-0.993.The results of HCA and PCA were consistent with those of SA. Conclusion: The method is fast and efficient. It can be used for the quality evaluation of Ginkgo leaves extract and Ginkgo leaves solid preparation.