Objective: To establish a HPLC fingerprint of Yixinshu tablet

in order to provide methods for the quality evaluation of Yixinshu tablet. Method: Sample was extracted with 75% methanol

detected with Waters Symmetry ShieildTM C18 column (4.6 mm×250 mm

5 μm)

and eluted with a mobile phase of acetonitrile-0.5%formic acid in a gradient mode. The flow rate was 1.0 mL·min-1

the detection wavelength was 260 nm

and the column temperature was 30℃. The chemical fingerprint similarity of 10 batches of Yixinshu tablet was calculated with the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012) published Chinese Pharmacopoeia Commission the standard substance comparison and HPLC-MS were adoped to identify the common peaks. Result: The fingerprint chromatography for the 10 batch of Yixinshu tablet included 29 common peaks

with a good separation at each peak. The relative retention times for common peaks of each batch were less than 1.0%

and the similarities among 10 samples were greater than 0.90.A total of 12 peaks were identified. Conclusion: Method is accurate

reproducible and stable

which can be used for the quality control of Yixinshu tablet.