Pharmacokinetics of Nine Components in Rat Plasma After Oral Administration of Qingqing Granules by LC-MS/MS

ZHAO Yan; ZHANG Mei; REN Ai-nong; WU Jie; HUANG Hou-cai; ZHONG Rong-ling; YANG Yu-lan; LI Jie

doi:10.13422/j.cnki.syfjx.2017010085

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Pharmacokinetics of Nine Components in Rat Plasma After Oral Administration of Qingqing Granules by LC-MS/MS

更新时间：2024-01-04

- Pharmacokinetics of Nine Components in Rat Plasma After Oral Administration of Qingqing Granules by LC-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 1, Pages: 85-90(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017010085
  CLC： R285.5
- Published：2017
- 稿件说明：
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ZHAO Yan, ZHANG Mei, REN Ai-nong, et al. Pharmacokinetics of Nine Components in Rat Plasma After Oral Administration of Qingqing Granules by LC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 85-90.
DOI：

ZHAO Yan, ZHANG Mei, REN Ai-nong, et al. Pharmacokinetics of Nine Components in Rat Plasma After Oral Administration of Qingqing Granules by LC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 85-90. DOI： 10.13422/j.cnki.syfjx.2017010085.

摘要

目的：建立同时测定大鼠血中9个成分（白芍苷、芍药苷、甘草苷、黄芩苷、盐酸小檗碱、汉黄芩苷、甘草酸、黄芩素、汉黄芩素）含量的方法，并将该方法用于清清颗粒的药代动力学研究。方法：采用Promosil C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.1%甲酸水溶液梯度洗脱，流速0.3 mL·min-1，柱温35℃；采用正离子模式；分为两段时间检测，前7 min只检测白芍苷、芍药苷和甘草苷，8~17 min检测其余化合物。结果：各成分的线性关系良好（r>0.998）；日间和日内精密度RSD均<15%；准确度的相对回收率90.58%~108.67%。9种成分的达峰时间（tmax）依次为（0.25±0），（0.25±0），（0.25±0），（0.25±0），（1.00±0.10），（6.33±0.82），（1.00±0.11），（0.25±0），（3.12±1.15）h，半衰期（t1/2）依次为（0.21±0.02），（6.72±2.10），（2.70±0.36），（6.90±1.55），（5.16±0.95），（4.34±1.02），（4.74±2.52），（4.79±1.48），（5.78±2.41）h，药峰浓度（Cmax）依次为（3.08±0.94），（10.01±2.17），（18.73±2.84），（36.18±7.57），（50.76±10.61），（793.70±214.08），（54.73±18.01），（207.35±27.58），（50.79±5.95）μg·L-1。结论：该方法简便、准确、重复性好，专属性强，可用于清清颗粒的药代动力学研究。

Abstract

Objective: A liquid chromatography-tandem mass spectrometric method (LC-MS/MS) was developed and validated for simultaneous determination of albiflorin

paeoniflorin

liquiritin

baicalin

berberine hydrochloride

wogonoside

glycyrrhizic acid

baicalein and wogonin in rat plasma with icariin as the internal standard. Method: Concentrations of the target analytes in plasma were determined by LC-MS/MS

chromatographic separation was accomplished on a Promosil C18 column (4.6 mm×250 mm

5 μm) with a mobile phase consisting of acetonitrile and 0.1%formic acid aqueous solution for gradient elution.Detection was performed in the multiple reaction monitoring mode with a positive ionization mode.Two time segments were performed in a single HPLC run

the first 7 minutes just scanned ions of albiflorin

paeoniflorin and liquiritin

the second time segment scanned ions of the remaining analytes. Result: All calibration curves showed good linearity (r>0.998) over a wide concentration range for all analytes.RSDs of intra-day and inter-day precisions were all within 15%and the relative recovery of accuracy were in the range of 90.58%to 108.67%.The peak time (tmax) of these nine components were (0.25±0)

(0.25±0)

(1.00±0.10)

(6.33±0.82)

(1.00±0.11)

(0.25±0)

(3.12±1.15) h

respectively;the half life (t1/2) of them were (0.21±0.02)

(6.72±2.10)

(2.70±0.36)

(6.90±1.55)

(5.16±0.95)

(4.34±1.02)

(4.74±2.52)

(4.79±1.48)

(5.78±2.41) h

respectively;the peak concentration (Cmax) of them were (3.08±0.94)

(10.01±2.17)

(18.73±2.84)

(36.18±7.57)

(50.76±10.61)

(793.70±214.08)

(54.73±18.01)

(207.35±27.58)

(50.79±5.95) μg·L-1

respectively. Conclusion: The validated method is successfully applied to pharmacokinetics of nine components in rats after oral administration of Qingqing granules.

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