Effect of Taohong Siwutang on Contents of ET-1, Ang-1 and VEGF in Serum of Cerebral Ischemia Rats

WANG Fei-long; HAN Lan; FAN Ling; WANG Meng-meng; GUO Yu-qi; GUO Dong-dong; WU Huan-ru; PENG Dai-yin

doi:10.13422/j.cnki.syfjx.2017010101

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Effect of Taohong Siwutang on Contents of ET-1, Ang-1 and VEGF in Serum of Cerebral Ischemia Rats

更新时间：2024-01-04

- Effect of Taohong Siwutang on Contents of ET-1, Ang-1 and VEGF in Serum of Cerebral Ischemia Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 1, Pages: 101-106(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017010101
  CLC： R285.5
- Published：2017
- 稿件说明：
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WANG Fei-long, HAN Lan, FAN Ling, et al. Effect of Taohong Siwutang on Contents of ET-1, Ang-1 and VEGF in Serum of Cerebral Ischemia Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 101-106.
DOI：

WANG Fei-long, HAN Lan, FAN Ling, et al. Effect of Taohong Siwutang on Contents of ET-1, Ang-1 and VEGF in Serum of Cerebral Ischemia Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 101-106. DOI： 10.13422/j.cnki.syfjx.2017010101.

摘要

目的：观察实验性脑缺血损伤大鼠血清中内皮素1（ET-1），血管形成素1（Ang-1）和血管内皮生长因子（VEGF）水平的变化及桃红四物汤（TSD）对其的干预作用。方法：健康SD大鼠48只，以线栓法构建大鼠大脑中动脉栓塞致局灶性缺血损伤模型，实验动物随机分为6组，分别为假手术组、模型组、桃红四物汤3个剂量组（18，9，4.5 g·kg-1）和尼莫地平组（0.02 g·kg-1）。连续ig 7 d，采用TTC染色法对脑梗死体积进行检测，通过测定全血黏度和血浆黏度，观察桃红四物汤对脑缺血损伤大鼠血液流变学的影响，酶联免疫吸附测定（ELISA）检测大鼠血清中ET-1，Ang-1和VEGF水平。结果：桃红四物汤高剂量组能显著减小脑缺血梗死体积。与假手术组比较，模型组全血黏度高、中、低切及血浆黏度均升高，差异具有统计学意义；与模型组比较，桃红四物汤高、中剂量组可显著降低高、中、低3个切变率下的全血黏度及血浆黏度，桃红四物汤低剂量组可显著降低脑缺血大鼠的血浆黏度；与模型组比较，桃红四物汤能显著降低血清中ET-1含量，显著升高血清中Ang-1和VEGF含量。结论：桃红四物汤可以明显改善脑梗死情况以及促进实验性脑缺血大鼠缺血区的血管新生，其作用机制可能与调控脑缺血大鼠血清中ET-1，Ang-1，VEGF含量有关。

Abstract

Objective: To observe the effect of Taohong Siwutang (TST) on the contents of endothelin-1 (ET-1)

angiopoietin-1 (Ang-1) and vascular endothelial growth factor (VEGF) in serum of cerebral ischemia rats. Method: Models of cerebral ischemia were reproduced with 48 healthy SD rats through middle cerebral artery occlusion (MACO). All rats were divided into six groups:sham group

model group

Taohong Siwu decoction groups (18

4.5 g·kg-1) and nimodipine group (0.02 g·kg-1). After the successive oral administration for 7 days

the volume of cerebral infarction was detected by TTC staining method; And whole blood viscosity and plasma viscosity were tested to observe the effects of TST on the hemorheology of rats with cerebral ischemia injury; And the content of serum ET-1

Ang-1 and VEGF were detected by ELISA. Result: The high dose group of TST could significantly reduce cerebral infarction volume. Compared with the sham group

whole blood viscosity and plasma viscosity of rats in model group were significantly increased

with no statistical significance. Compared with the model group

high and medium-dose TST treatment groups showed significant decreases in whole blood viscosity and plasma viscosity. Meanwhile

the low-dose TST treatment group showed significant decrease in the plasma viscosity in comparison with model group. Compared with the model group

TST remarkably reduced the levels of ET-1 in serum

and increased the levels of Ang-1

VEGF in serum. Conclusion: TST has the effect in markedly improving cerebral infarction volume

and promoting angiogenesis in cerebral ischemia rats. Its mechanism may be related to the regulation of content of ET-1

Ang-1 and VEGF in serum.

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