Content Determination of Loganic Acid and Gentiopicroside During Process of Seed Germination in

QIAN Chun-sheng; ZHAO Zhi-li; CHENG Xue-mei; WU Jin-rong

doi:10.13422/j.cnki.syfjx.2017020023

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Content Determination of Loganic Acid and Gentiopicroside During Process of Seed Germination in

更新时间：2024-01-04

- Content Determination of Loganic Acid and Gentiopicroside During Process of Seed Germination in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 2, Pages: 23-26(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017020023
  CLC： S567.239
- Published：2017
- 稿件说明：
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QIAN Chun-sheng, ZHAO Zhi-li, CHENG Xue-mei, et al. Content Determination of Loganic Acid and Gentiopicroside During Process of Seed Germination in [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(2): 23-26.
DOI：

QIAN Chun-sheng, ZHAO Zhi-li, CHENG Xue-mei, et al. Content Determination of Loganic Acid and Gentiopicroside During Process of Seed Germination in [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(2): 23-26. DOI： 10.13422/j.cnki.syfjx.2017020023.

摘要

目的：探究粗茎秦艽种子萌发过程中马钱苷酸和龙胆苦苷的含量变化。方法：建立粗茎秦艽种子中马钱苷酸和龙胆苦苷高效液相色谱（HPLC）含量测定方法：Cat C18色谱柱（4.6 mm×150 mm，3.5 μm），流动相为乙腈（A）-0.04%乙酸水（B），梯度洗脱，流速0.8 mL·min-1，检测波长为254 nm，柱温为30℃，进样量为5 μL。测定种子不同萌发时期马钱苷酸和龙胆苦苷的含量。结果：马钱苷酸和龙胆苦苷的线性范围分别为35.5~761.5 mg·L-1（r1=1.000 0），4.8~103.1 mg·L-1（r2=0.999 9），线性关系良好，平均加样回收率分别为98.30%（RSD 1.9%），99.85%（RSD 2.6%）；粗茎秦艽种子在萌发过程中，马钱苷酸的含量呈不断降低的趋势，龙胆苦苷的含量呈不断上升的趋势。结论：粗茎秦艽种子萌发过程中，龙胆苦苷和马钱苷酸的含量动态变化呈负相关性趋势，且相关性程度与种子的发芽率有关。

Abstract

Objective: To determine the contents of loganic acid and gentiopicroside during seed germination in Gentiana crassicaulis. Method: HPLC method for simultaneous determination of loganic acid and gentiopicroside during seed germination in G. crassicaulis. The separation was done on Cat C18 (4.6 mm×150 mm

3.5 μm) column with eluted with acetonitrile (A)-0.04% acetic acid solution (B) as the mobile phase for gradient elution. The flow rate was 0.8 mL·min-1. The detect wave length was set at 254 nm. The column temperature was kept at 30℃

and the sample volume was 5 μL to determine the contents of loganic acid and gentiopicroside during seed germination. Result: Loganic acid and gentiopicroside were well separated with ideal linear correlations within the range of 35.5~761.5 mg·L-1 (r1=1.000 0) and 4.8~103.1 mg·L-1 (r2=0.999 9) respectively. The average recoveries were 98.30% (RSD 1.9%) and 99.85% (RSD 2.6%) respectively. During germination

the content of loganic acid rose and the content of gentiopicroside declined gradually. Conclusion: A negative correlation of loganic acid and gentiopicroside is present during seed germination of G. crassicaulis

and the correlation degree is related to seed germination percentage.

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