Objective: To provide a basis for the development

utilization and qualitycontrol of Phytolacca Radix through quality and fingerprint analysis of Phytolacca Radix from different sources in Henan. Method: Thin-layer chromatography was used for qualitative identification; HPLC-ELSD method was used for the determination of esculentoside A content with moble phase of methanol-4% acetic acid (30:70); the flow rate was 1.0 mL·min-1 and column temperature was 30℃

evaporative light scattering detector (ELSD) was used for detection:gas flow rate of 2.0 L·min-1

drift tube temperature of 90℃

record time of 20 min and the sample size of 20 μL. Fingerprint analysis was conducted by using HPLC-ELSD and gradient elution

with a gas flow rate of 2.0 L·min-1

drift tube temperature of 105℃. Result: There was difference in content of esculentoside A of the samples from different areas

and the content was higher between 1.29%-3.52% in samples from Jiyuan

Xixia and Lushan; acid-insoluble ash content was qualified in all samples. There were 9 common peaks in the fingerprint

and peak 7 was the reference peak. Conclusion: The results of this study can be used as the reference for the formulation of Phytolaccae Radix quality standard.