Influence of Compatibility of Aconiti Radix and Pinelliae Rhizoma on CYP3A in Rats

CHEN Hong; CHENG Zai-xing; WU Jin-jun; LIN Na

doi:10.13422/j.cnki.syfjx.2017020075

您当前的位置：

首页 >

文章列表页 >

Influence of Compatibility of Aconiti Radix and Pinelliae Rhizoma on CYP3A in Rats

更新时间：2024-01-04

- Influence of Compatibility of Aconiti Radix and Pinelliae Rhizoma on CYP3A in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 2, Pages: 75-80(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017020075
  CLC： R285.5
- Published：2017
- 稿件说明：
移动端阅览
CHEN Hong, CHENG Zai-xing, WU Jin-jun, et al. Influence of Compatibility of Aconiti Radix and Pinelliae Rhizoma on CYP3A in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(2): 75-80.
DOI：

CHEN Hong, CHENG Zai-xing, WU Jin-jun, et al. Influence of Compatibility of Aconiti Radix and Pinelliae Rhizoma on CYP3A in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(2): 75-80. DOI： 10.13422/j.cnki.syfjx.2017020075.

摘要

目的：研究川乌配伍半夏对大鼠细胞色素P450酶系（CYP）3A1/2的影响，从CYP介导的代谢性相互作用角度阐述“十八反”中半夏反乌头的科学内涵。方法：采用体外温孵法和体内探针法检测CYP3A活性，采用免疫印迹试验（Western blot）和荧光定量聚合酶链式反应技术检测蛋白和mRNA表达。结果：与生/制川乌配生半夏对应剂量组相比，生/制川乌配法/姜半夏组6β-羟基睾酮（6β-OH-Tes）生成速率显著降低；生川乌配法/姜半夏组的CYP3A蛋白表达明显降低，制川乌配法/姜半夏仅部分剂量组明显降低；生川乌配法/姜半夏在0.6和1.2 g·kg-1剂量组CYP3A1 mRNA表达显著增加，制川乌配法/姜半夏在0.60 g·kg-1剂量组CYP3A1 mRNA表达显著降低。与生川乌配生/法/姜半夏对应剂量组相比，制川乌配生/法/姜半夏组6β-OH-Tes生成速率亦多显著降低；制川乌配生/法半夏组CYP3A蛋白表达明显降低；制川乌配生/法/姜半夏组CYP3A1 mRNA表达呈增加趋势。与生川乌配生半夏组相比，配伍法半夏后6'-羟基丁螺环酮（6'-OH-BP）和丁螺环酮（BP）的药时曲线下面积（AUC0-t）比值（6'-OH-BP/BP），1-（2-嘧啶基）哌嗪（1-PP）与BP的AUC0-t比值（1-PP/BP）均增加，而配伍姜半夏后二者生成未发生变化；与制川乌配生半夏相比，配伍法/姜半夏后6'-OH-BP/BP均减小，而1-PP/BP均未变。结论：配生/法/姜半夏后均可逆转生/制川乌对大鼠CYP3A的抑制作用，可加快川乌中相应成分的代谢，起到降毒或减效作用。

Abstract

Objective: The aim of the article was to explore the compatibility connotation of Aconiti Radix and Pinelliae Rhizoma from cytochrome P450 enzyme system (CYP)3A1/2 mediating metabolic herb-herbs interaction in rats. Method: Incubation in vitro and probe method in vivo were used to detect the activity of CYP3A.The fluorescence quantitative polymerase chain reaction (PCR) technology and Western blot were applied to determine expression of mRNA and protein. Result: Compared with unprocessed Aconiti Radix (RAR)/Aconiti Radix Cocta (ARC)-unprocessed Pinelliae Rhizoma (UPR)

the production rate of 6β-hydroxy-testosterone (6β-OH-Tes) and the expression of CYP3A protein were all decreased in RAR-Pinelliae Rhizoma Praeparatum (PRP)/Pinelliae Rhizoma Praeparatum Cum Zingibere et Alumine (PRPZA)

but partial dose group of ARC-PRP/PRPZA was significantly decreased.The expression of CYP3A1 mRNA were increased in 0.6

1.2 g·kg-1 groups of RAR-PRP/PRPZA as well as decreased in 0.6 g·kg-1 group of ARC-PRP/PRPZA.Compared with the corresponding dose group of RAR-UPR/PRP/PRPZA

the production rates of 6β-OH-Tes were most significantly reduced and the expression of CYP3A protein of ARC-UPR/PRP were decreased

while the expression of CYP3A1 mRNA were increased in most groups of ARC-UPR/PRP/PRPZA.The rates of 6'-hydroxy-buspirone (6'-OH-BP)/buspirone (BP) (AUC0-t of 6'-OH-BP/AUC0-t of BP) and 1-(2-pyrimidinyl)-piperazine (1-PP)/BP (AUC0-t of 1-PP/AUC0-t of BP) were all increased in RAR-PRP as well as did not change in RAR-PRPZA by comparing with RAR-UPR.The rates of 6'-OH-BP/BP were all decreased and the rates of 1-PP/BP did not change in ARC-PRP/PRPZA by comparing with ARC-UPR. Conclusion: Inhibition of RAR/ARC on CYP3A activity can be reversed by compatibility with UPR/PRP/PRPZA

which may accelerate the metabolism of some components in RAR/ARC

then the toxicity or the pharmacological action of RAR/ARC is weakened.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Influence of Wutoutang Adding Different Processed Products of Pinelliae Rhizoma on CYP3A in Rats

Effect of Shenge Bushen Capsules and Its Polysaccharides and Flavonoids on Precocious Puberty in Young Mice

Structural Characteristics and Antioxidant Activity Analysis of Polysaccharides from Pinelliae Rhizoma and Its Processed Products Before and After Hydrolysis （Enzymolysis） by Sugar Spectrum

Medication Rules of Pinelliae Rhizoma Processed Products in Syndrome Differentiation and Treatment of Insomnia Based on Data Mining

Identification of Arisaematis Rhizoma and Pinelliae Rhizoma by PCR-RFLP

Related Author

SUN Hong

LEI Fan

LI Chenggong

HU Shixian

WANG Weihua

REN Bin

HAO Juan

LUO Rui

Related Institution

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

School of Pharmacy，Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province，Chengdu University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰