Objective: To observe the effects of Zhali Nusi Fang on macroscopic syndrome

hemorheological parameters and brain cell apoptosis in cerebral ischemia reperfusion of rats with Qi deficiency and blood stasis. Method: Totally 170 rats were randomly divided into normal group

sham operation group

model group

nimodipine group

high

medium and low-dose Zhali Nusi Fang groups (29.2

14.6

7.3 g·kg-1). Expect for normal group and sham operation group

all of the remaining groups were subdivided into 3-day and 7-day groups

with 15 in each group. Except for normal group

all of the remaining groups were included into the Qi deficiency and blood stasis mode by means of combined factors of hunger

tiredness

cold dampness

panic and high fat diet for 4 weeks. Drugs were given to the rats through intragastric administration for 4 days. The middle cerebral artery occlusion (MCAO) model was duplicated to observe the rats' nerve mental status

changes in body weight

feeding

tongue and defecation. Blood was collected for hemorheology detection; Tunel method was used to detect apoptosis of brain cortex. Result: Compared with the normal group

according to macroscopic observation and detection of blood rheology indexes in the sham operation group

the syndrome of qi deficiency and blood stasis syndrome was observed successfully. Compared with the sham operation group

the model group showed obvious hemiplegia symptoms in addition Qi deficiency blood stasis symptoms

and neurological scores of rats in model group significantly increased (P<0.01)

whole blood viscosity significantly rose (P < 0.05

P < 0.01)

and changes in blood rheology indexes were significantly reduced (P<0.05)

blood cell aggregation index and hematocrit significantly increased (P<0.01)

and nerve cell apoptosis was obvious (P<0.01). Compared with the model group

Zhali Nusi Fang can significantly improve cerebral ischemia and reperfusion rats with Qi deficiency and blood stasis

and decrease whole blood viscosity

hematokrit

blood cell aggregation

improve the deformation of red blood cells

and inhibit the apoptosis of brain cells

especially in the high-dose 7d group. Conclusion: Zhali Nusi Fang has a good protective effect on cerebral ischemia and reperfusion in rats with Qi deficiency and blood stasis

its mechanism may be closely related to improvement of blood rheology and inhibition of brain nerve cell apoptosis of model rats.